Source: //www.biomerieux.com/corp/en/resource-hub/knowledge/white-papers/pharmaceutical-qc-white-papers/automated-1-hour-mycoplasma-testing-in-bioproduction.html

Delivering Precise, Accurate, and Cost-efficient Liquid Transfers

The Echo Liquid Handler has revolutionized liquid handling with an innovative technology that produces faster and more accurate results than traditional pipetting methods. Whether you are involved in drug discovery, compound management, genomics research, synthetic biology, proteomics, functional screening, or other research applications, you can do better science and save money with the Echo acoustic droplet ejection (ADE) technology.

Powerful but gentle in its approach, ADE drives Echo Liquid Handlers. It focuses ultrasonic acoustic energy at the meniscus of a fluid sample to eject small droplets of liquid from wells and position them precisely onto a surface suspended above the ejection point.

Echo Acoustic Liquid Handling

The Echo Liquid Handler revolutionizes liquid handling by using acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source. The Echo Liquid Handler can transfer as many as 750,000 samples a day to 384-, 1536-, and 3456-well plates, slides, and microfluidic devices, without touching the samples. This groundbreaking technology benefits laboratory workflows and operations of all sizes.

DYNAMIC FLUID ANALYSIS

On-the-Fly Adjustments to Changing Fluid Properties

Dynamic Fluid Analysis enables Echo Liquid Handlers to adapt easily to different types of fluids. This patented software algorithm:

• Adjusts acoustic energy requirements in real time without operator intervention
• Eliminates manual calibration
• Ensures accurate, precise fluid transfers
• Increases the range of fluids transferred by acoustic energy

The Echo Liquid Handler uses Dynamic Fluid Analysis capabilities to simplify experimental setup and enables a higher degree of experimental and workflow flexibility.

Key Benefits

• No need to calibrate the instrument in advance �?the Echo Liquid Handler determines transfer parameters for each reagent at runtime.
• Larger-volume transfers are accomplished by transferring the same drop size repeatedly—there’s no requirement to scale with volume.
• Dynamic Fluid Analysis is an active process—and therefore less sensitive to reagent changes than a single calibration point—so downstream experimental data can be more consistent.
• Dynamic Fluid Analysis determines the appropriate transfer parameters on a well-by-well basis, and thus works with more complex reagent sets or inconsistent reagents.

Background on Fluid Transfer and Liquid Handlers

Fluid transfer methods are impacted by two key fluid properties: surface tension (the way fluid at an interface interacts with its surroundings) and viscosity (the resistance of fluid to move). Most liquid handlers are passive and require operators to determine how to overcome surface tension and viscosity for their specific reagents, putting the burden on them to calibrate the instrument to transfer that reagent.

This burden is substantial, because calibrations:

• Must be verified and repeated on every instrument that is required to transfer that reagent.
• Usually work over only a limited volume range.
• May drift with the age of the liquid handler as mechanical components wear down.
• Are only an approximation; calibrations against inconsistent reagents (e.g., cell lysates in a screen, or high-viscosity reagents) may not transfer accurately and precisely, which may lead to data errors downstream.
• Cannot be developed for certain types of reagents on multichannel liquid handlers (e.g., commercially available crystallography reagents, by design, consist of reagents of varying viscosity and surface tension properties).

Dynamic Fluid Analysis Technology Improves Liquid Handling

Echo Liquid Handlers use sound energy to transfer reagents in drop increments on a 2.5 or 25 nL scale (instrument dependent). All models incorporate a transducer, which converts electrical energy to sound energy to accomplish the drop transfer. The transfer process can be explained in two broad steps:

SURVEY: The Echo Liquid Handler determines fluid height and properties in the source wells. The transducer (acoustic energy generator) sends soft energy pings into each well and receives the reflections (or echoes) returned from three interfaces:

• The bottom of the source microplate
• The bottom of the well
• The fluid meniscus

TRANSFER: The transducer returns to the first transfer point and sends a larger energy burst to accomplish the transfer in discrete, consistent droplets. Key to this process is that the transducer interfaces with one well at a time. This affords the capability to perfect transfer for each source well. General information on the fluid type provided by the operator (e.g., DMSO, buffer, reagent containing glycerol, etc.) narrows the transfer parameters.

Dynamic Fluid Analysis uses a power-adjustment ramping process to slowly increase the energy from survey power level to transfer power levels. By listening to subsequent echoes, the Echo Liquid Handler can determine the appropriate power level required for transfer from each source well. This process is accomplished in milliseconds and requires no operator intervention.

Source: //www.mybeckman.vn/liquid-handlers/echo-acoustic-technology

Our products: //gd-1.com/en/product-category/biological-technology/biological-analysis-instrument/automated-sample-preparation/acoustic-liquid-handlers/

The Digital Classroom solution with smart microscopes from ZEISS provides the foundation for implementing digital interactive classrooms, providing an intuitive teaching and learning experience, which helps to promote the passion for scientific discovery among students.

The Digital Classroom solution is a Wi-Fi enabled microscope system that can connect to Instructors and students’ iPads, iPhones or Windows-PCs, allowing Instructors to use document sharing features, slideshows and many other useful tools to make practical lessons more interesting and engaging than ever.

Minh Khang Co., Ltd will organize a completely free Demo Workshop series to introduce the “Digital Classroom Solution” with the ZEISS microscope system.

If you are interested in this solution, please contact Minh Khang Company for advice and support, or leave information in the following form:

//forms.gle/46KddwpjLWR8WGpM7

Leica Biosystems, founded in 1989, is one of the world’s leading companies providing comprehensive solutions for instruments, chemicals and consumables in pathology, from biopsy to diagnosis. .

Leica Biosystems is a member of the Danaher group, with headquarters in Germany and operations in more than 100 countries. The mission of “Enhancing cancer diagnosis, improving lives�?is at the heart of the development of Leica Biosystems.

This is our honor to become the exclusive distributor in the South of Vietnam of Leica Biosystems!

Selective media are designed to provide a conducive environment for growth of some types of microorganisms but not to others. They provide the ability to control what will and will not grow on a culture plate. However, it is important to note that a microorganism will not grow as well on a selective media compared to a non-selective media. This is entirely due to non-selective media being well balanced with all the elements that most bacteria need to grow and flourish, whereas in selective media, due to the presence of either activating or inhibiting elements, selected microorganism will grow but not thrive.

Therefore, compendial test requirements for selective media are not quantitatively evaluated. Instead, pharmacopeia requirements for selective media testing state:

Growth of the microorganism comparable to that previously obtained with a previously tested and approved batch of medium occurs.

No percent recovery requirement must be achieved when testing a selective media batch. The qualification is based on growth of the new batch of selective media comparable to growth of previously tested and approved batch of the same selective media.

Source: Biomerieux