timsTOF РC̫ng ty TNHH TM DV KT Minh Khang //gd-1.com Thu, 29 Jun 2023 06:43:42 +0000 en-US hourly 1 //wordpress.org/?v=6.1.4 //gd-1.com/wp-content/uploads/2021/02/favicon-32x32.png timsTOF РC̫ng ty TNHH TM DV KT Minh Khang //gd-1.com 32 32 timsTOF РC̫ng ty TNHH TM DV KT Minh Khang //gd-1.com/en/san-pham/timstof-ultra/ Wed, 28 Jun 2023 07:09:52 +0000 //gd-1.com/?post_type=product&p=8639

Unmatched sensitivity with timsTOF Ultra and CaptiveSpray Ultra

Discover the extraordinary capabilities of the timsTOF Ultra mass spectrometer and CaptiveSpray Ultra ion source for groundbreaking omics research. With their combined force, they unleash unparalleled sensitivity, allowing you to conquer samples, detect low-abundance peptides, and make groundbreaking discoveries.

Unlock the hidden secrets: From minute samples to unseen peptides
The timsTOF Ultra and CaptiveSpray Ultra offer a formidable alliance that unlocks hidden secrets in minute samples and unseen peptides. Through the exceptional power of PASEF® acquisition, you can delve into the depths of low-abundance peptides and reveal previously undiscovered information. The incorporation of trapped ion mobility separation eliminates the need for chemical tagging, making the identification of novel peptides effortless. With these advancements, the timsTOF Ultra empowers researchers to redefine what is possible within their labs.

Break boundaries
The timsTOF Ultra transcends conventional limitations, opening doors to new scientific possibilities. Whether it’s single cell proteomics, immunopeptidomics, or plasma proteomics, this instrument delivers unmatched speed and sensitivity. By harnessing the capabilities of the timsTOF Ultra, researchers can push the boundaries of their work and achieve groundbreaking results.

]]> timsTOF РC̫ng ty TNHH TM DV KT Minh Khang //gd-1.com/en/san-pham/timtof-ht/ Wed, 28 Jun 2023 06:53:48 +0000 //gd-1.com/?post_type=product&p=8636

TIMS based ion mobility separation and CCS-enabled analysis

The new timsTOF HT mass spectrometer is equipped with the 4th generation high capacity TIMS-XR analyzer and advanced digitizer technology (ADT). It offers higher dynamic range for unmatched analytical depth and quantitation in high-throughput proteomics experiments. The timsTOF HT is fully CCS-enabled and supports all PASEF® acquisition modes including PASEF®, dia-PASEF® and prm-PASEF® achieving flexibility in 4D-Proteomics� class=. Simply uncompromised proteomic depth at scale.

]]> timsTOF РC̫ng ty TNHH TM DV KT Minh Khang //gd-1.com/en/san-pham/timstof-maldi-pharmapulse/ Wed, 28 Jun 2023 04:37:54 +0000 //gd-1.com/?post_type=product&p=8633

Unbiased, deep HTS by label-free mass spectrometry

The timsTOF MPP represents the flagship member of the Bruker PharmaPulse® product line. It features the extreme speed and proven robustness of MALDI and, for the first time ever in HTS, takes advantage of Bruker´s innovative Trapped Ion Mobility Spectrometry (TIMS) technology. TIMS enables rapid gas-phase separation of isobars, and even isomers, by exploiting the molecular collisional cross-section. This, in combination with routine 50,000 mass resolution in QTOF-MS detection enables revolutionary levels of assay specificity at HTS speed. timsTOF MPP uses a dual MALDI / ESI ion source with industry-leading 10 kHz smartbeam� class= 3D laser to enable uHTS compatible speed and throughput, and is available with the unique MALDI-2 option for an expanded chemical space.

As part of the solution, the new MALDI PharmaPulse® 2023 software supports a broad range of HTS applications for drug discovery. Its automation interface allows for timsTOF MPP integration in high-throughput environm

Exceptional data quality delivered at uncompromised HTS speed

ents and works in concert with scheduling software from various vendors. Furthermore, MPP 2023 enables seamless transfer of data and results to downstream analysis software, e.g., to Genedata Screener®.

timsTOF MALDI PharmaPulse
]]> timsTOF РC̫ng ty TNHH TM DV KT Minh Khang //gd-1.com/en/san-pham/timstof-scp/ Wed, 28 Jun 2023 04:17:19 +0000 //gd-1.com/?post_type=product&p=8630 Redefining single cell proteomics

Advanced ion optics and PASEF to investigate cell heterogeneity and biology from a single cell
Mass spectrometric proteomics has become a staple of modern research in understanding biological function and disease mechanisms. Healthy or diseased tissues that seem homogenous are composed of cells with a variety of different proteomes. The challenge of deciphering the proteomes in each single cell �the cell heterogeneity �holds the key to fully understanding its function.
The timsTOF SCP offers a radically improved ion source concept. Combined with parallel accumulation serial fragmentation (PASEF®) acquisition methods, it provides extremely high speed and sensitivity to tackle proteomes of single cells or post translational modifications in a few cells that are morphologically or functionally similar.

Radically improved ion transfer

The timsTOF SCP features a modified ion source geometry that includes 1 mm capillary identification for five times higher ion transfer into an additional higher pressure stage ion funnel and 8-stage differentially pumped vacuum system.

The larger capillary yields ultra-high sensitivity and the additional orthogonal ion reflection and subsequent funnel offers a separate, differentially pumped stage, maintaining the system robustness expected from the timsTOF instrument series.

Dramatic improvements in proteomics performance

The timsTOF SCP offers improvements in ion transmission into the source while maintaining robustness with an added higher pressure vacuum stage. This results in an almost 5x improvement in ion current. When combined with the Evosep One Whisper method running at 100 nL/min flow rate, and the dia-PASEF method, sensitivity gains of about 100X over previous high-flow results with the Evosep One are achieved. This enables unbiased proteomics at the true single cell level with good reproducibility, robustness and coverage of about 1500 proteins per cell for the first time.

Dual-TIMS, CCS-enabled analysis

Trapped ion mobility spectrometry (TIMS) is first and foremost a separation technique in the gas phase. This resolves sample complexity through an added dimension of separation in addition to high performance liquid chromatography (HPLC) and mass spectrometry, increasing peak capacity and confidence in compound characterization.

Equally important, the TIMS device also accumulates and concentrates ions of a given mass and mobility, enabling a unique increase in sensitivity and speed. A near 100% duty cycle can be achieved with the dual-TIMS technology facilitating accumulation in the front section, while ions in the rear section are sequentially released depending on their mobility. This process of parallel accumulation serial fragmentation (PASEF®) enables collisional cross section (CCS) analysis.

CCS-enabled analysis opens up many further analytical possibilities, from greater certainty of compound identification to confident library matching and lower false discovery rates (FDRs) in large datasets.

Ideal for immunopeptidomics and other enrichment workflows

Besides unbiased true single cell proteomics applications, the timsTOF SCP also offers outstanding sensitivity for workflows that involve enrichment of peptides from the proteome. Immunopeptidomics studies start with purification of immunopeptides from plasma or tissue.

Since immunopeptides are present at relatively low abundance in these samples, the timsTOF SCP is ideal for immunpeptidomics for neo-antigen discovery where the available material is limited, as in needle biopsies. The timsTOF SCP also has the sensitivity to revolutionize the use of phosphoproteomics for the study of signaling pathways in cancer.

PASEF®

Peptide ions are separated using trapped ion mobility spectrometry (TIMS), eluted (~ 100 ms) and detected in the quadropole time of flight (QTOF), generating the TIMS MS heat map. In the PASEF® method the same TIMS separation is used with the quadrupole isolating a certain ion species during its elution and immediately shifting to the next precursor. Parent and fragment spectra are aligned by mobility values.

The parallel accumulation serial fragmentation (PASEF®) technology achieves a sequencing speed of >100 Hz. Using PASEF® increases the MS/MS spectra quality of the low abundant peptides by selecting them several times.

PASEF®: the perfect fit for shotgun proteomics
The timsTOF SCP powered by PASEF® offers a sequencing speed of >100 Hz without losing sensitivity or resolution. This is achieved by synchronizing the quadrupole isolation mass window with the elution time of the specific peptide packages from the TIMS funnel as well as the collision energy in the collision cell.

 

]]> timsTOF – Công ty TNHH TM DV KT Minh Khang //gd-1.com/en/san-pham/timstof-pro-2/ Wed, 28 Jun 2023 04:04:24 +0000 //gd-1.com/?post_type=product&p=8627 ®) technology, paves the way for 4D-Multiomics applications. With its proven robustness, high sensitivity and fast MS/MS acquisition, timsTOF Pro 2 is the platform of choice for 4D-Proteomics, 4D-Metabolomics and 4D-Lipidomics.]]> The new standard for high speed, high sensitivity 4D-Multiomics

Liquid chromatography (LC) coupled with mass spectrometry (MS) has become the gold standard in various omics fields. However, the coverage of proteomes or metabolomes in complex biological samples remains challenging due to limited speed, sensitivity and resolution of current mass spectrometers.

Adding trapped ion mobility spectrometry (TIMS) to the equation unlocks the parallel accumulation serial fragmentation (PASEF®) acquisition method to provide extremely high MS/MS speed and sensitivity, requiring minimal sample amounts.

Dual-TIMS and CCS-enabled analysis

TIMS is first and foremost a separation technique in the gas phase. This resolves sample complexity through an added dimension of separation in addition to high performance liquid chromatography (HPLC) and mass spectrometry, increasing peak capacity and confidence in compound characterization.

Equally important, the TIMS device also accumulates and concentrates ions of a given m/z and mobility, enabling a unique increase in sensitivity and speed.

A near 100% duty cycle can be achieved with the dual-TIMS technology facilitating accumulation in the front section, while ions in the rear section are sequentially released depending on their mobility. This process of parallel accumulation serial fragmentation (PASEF®) enables collisional cross section (CCS) analysis.

CCS-enabled analysis opens up many further analytical possibilities, from greater certainty of compound identification to confident library matching and lower false discovery rates (FDRs) in large datasets.

PASEF®

The timsTOF Pro 2 comes with our innovative dual-TIMS analyzer that offers three times higher ion capacity. Simplified ion optics maximize ion transfer and sensitivity to set a new standard in 4D-Multiomics. PASEF® is a redesigned MS/MS technology to meet speed requirements of omics applications. Ions are separated using trapped ion mobility spectrometry, eluted (~ 100 ms) and detected in the quadrupole time of flight (QTOF), generating the TIMS MS heat map. PASEF® then uses the same TIMS separation to serially fragment ions. The quadrupole isolates a certain ion species during its elution for MS/MS and immediately shifts to the next precursor. Parent and fragment spectra are aligned by mobility values. The timsTOF Pro 2 can achieve a sequencing speed of > 120 Hz in PASEF® mode. Furthermore, the MS/MS spectra quality of low abundant analytes can be enriched by selecting them several times.

 

]]> timsTOF РC̫ng ty TNHH TM DV KT Minh Khang //gd-1.com/en/san-pham/maldi-2-timstof-flex/ Wed, 28 Jun 2023 03:24:12 +0000 //gd-1.com/?post_type=product&p=8622 A second laser is available for post-ionization

The overall low ion yield and sensitivity issues due to ion suppression, using traditional MALDI, make the imaging analysis of samples sometimes very challenging. Our answer to these problems is MALDI-2. The post-ionization technique reduces ion suppression effects and improves sensitivity by orders of magnitude.

After the initial MALDI process, a second laser, sitting parallel to the sample surface, fires into the evolving plume and post-ionizes neutral (mainly matrix) molecules. A charge transfer from post-ionized matrix molecules to neutral analyte molecules leads to an amazing sensitivity gain for many analytes.

MALDI-2 and microGRID – A powerful combination making the possibility of sub-cellular imaging within reach

The need for uncompromising post-ionization has never been greater. High spatial resolution below 10 µm results in a drastically decrease of ablated and ionized sample material. MALDI-2 post-ionization compensates the lower sample amount per pixel, making even the finest molecular distributions visible.

Combining MALDI-2 post-ionization with microGRID enabled high spatial resolution opens the field for the analysis of the smallest unit of eukaryotic life – single cells. This unique combination makes the timsTOF fleX MALDI-2 the platform of choice for out of the box single cell imaging.

Analyze cell structures that are otherwise only visible with a microscope and obtain molecule-specific information with (sub)cellular resolution.
Complementing microGRID imaging with MALDI-2 post-ionization enhances the sensitivity detecting even the finest molecular distributions.

Post-ionization is easily accessible via software controlled solutions

timsTOF fleX MALDI-2 Hậu ion hóa

The post-ionization laser is built as an add-on to the timsTOF fleX. All functions are exactly the same on the timsTOF fleX MALDI-2. This instrument is build on the latest timsTOF HT technology and combines the most advanced Omics analyses with high sensitive MALDI operation. No physical hardware changes are needed to activate post-ionization.

By just one click in the software the user will be able to change between post-ionization and traditional MALDI mode. This is done in seconds and allows for direct comparison of the two different ionization processes. You do not have to be a professional MALDI operator to use MALDI-2 as it is easy to handle and therefore also suitable for beginners.

]]> timsTOF РC̫ng ty TNHH TM DV KT Minh Khang //gd-1.com/en/san-pham/timstof-flex/ Wed, 28 Jun 2023 02:48:23 +0000 //gd-1.com/?post_type=product&p=8616

microGRID – Accurate and robust high-resolution imaging made simple

High resolution imaging has never been easier. Bruker’s microGRID technology combines MALDI stage and smartbeam 3D laser beam positioning to facilitate high quality imaging down to 5 µm spatial resolution.

The optionally available microGRID for all timsTOF fleX instruments is fully integrated into Bruker’s established workflows to enable out of the box high spatial resolution imaging. The technology seamlessly integrates in Bruker’s fully automated SCiLSâ„? class= autopilot workflow, making the technology attractive not only for experts but also for novelists as well as for routine applications.

In combination with SCiLSâ„? class= Lab Bruker rounds off the package and delivers a high-performance software to analyze high-resolution imaging data.

Enhanced resolution of rat testis images from 20 µm to 5 µm with microGRID imaging allows to access fine tissue structures of the organ.

From 4D-Omics to molecular imaging without stopping, changing, modifying or compromises

Dual source design to match PASEF® empowered LC-MS/MS identification with label-free spatial localization to decode the molecular make up of your sample

Built on the standard for shotgun proteomics, the timsTOF fleX combines best in class 4D-Omics with Bruker’s cutting edge MALDI Imaging technology, including smartbeam 3D laser optics to be a fast measurement all in one platform. A dual source instrument ideal for SpatialOMx®, timsTOF fleX conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform. No single instrument has before provided access to both essential capabilities for the most advanced Omics researchers.

During operation, software activation of the smartbeam 3D laser is the only change in the source region to change from ESI to MALDI in seconds. No complicated changeover means making zero compromises in productivity and the ability to move effortlessly from world class Omics identification and quantification workflows to creating high definition molecular maps of tissue sections to see what matters most.

Dig deeper by adding an additional dimension to your MALDI images

Since ions produced by MALDI and ESI travel the same path to the detector from the source, MALDI workflows can take advantage of the most advanced features found in the timsTOF HT, including trapped ion mobility separation (TIMS) based on the collisional cross section (CCS) of detected molecules. Tuning and calibration can be performed in ESI mode and used for the MALDI experiment for additional ease of optimization.

TIMS allows for the separation of molecules dependent on the shape of the ions. Ions are entering the dual TIMS device together with a gas stream and are accumulated in the first section by an electrical field. The actual separation takes place in the second part of the TIMS cartridge. Ions are eluted in a temporal and spatial fashion by lowering the electrical potential. Variable ramp speed and mobility range adaptation gives rise to optimizations for different classes of molecules allowing high flexibility for the user.

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