An ideal entry-level system for both inexperienced and occasional IEF users, the CSL-IEF is also versatile enough to meet the needs of laboratories with increased throughput requirements.

Features include:

• A high-capacity focusing tray that accommodates up to twelve IPG strips
• Adjustable pick-and-place electrodes clip conveniently anywhere within the focusing tray to resolve IPG strips 7-24cm in length; colour-coded to prevent polarity reversal
• A cooling plate, manufactured from a special grade ceramic in a large 26x26cm surface area, facilitates effective heat dissipation and homogenous thermal control, particularly during high voltage IEF techniques
• An optional, but recommended, Cleaver Scientific recirculating chiller connects quickly and easily to the cooling plate via snap-lock connectors to maintain optimal operating temperatures for IPG strips (20) and precast gels (4)
• Rehydration tray allows convenient transfer of IPG strips to the focusing tray without time-consuming removal of residual rehydration buffer; also enables focusing tray to remain permanently in use for IEF to maximise throughput, and provides useful storage at -20 for focused strips before second-dimension runs
• Electrode frame clips directly on to the cooling plate and includes adjustable electrodes to run horizontal precast IEF and PAGE gels

This unit has a 12 month limited warranty

• 5g Agaroza x 3
• TBE mattress (2 bags)
• Marker 1 KB x 2
• Runsafe DNA Dye

The Plunger has been carefully designed with a high quality spring mechanism to ensure snag free, soft movement. Simply turn it to adjust the instrument’s volume comfortably.

Use Various Tips with a Universal Tipcone

A Universal Tipcone enhances the compatibility of the instrument and enables it to easily work with most of the internationally accepted standard tips.

Set the Volume with Perfection

A soft click sound at every increment ensures perfect volume setting and prevents any accidental changes. It also facilitates single-handed operation.

Store Safely with a Holder

The instrument includes a Holder that enables easy, efficient and safe storage.

Eject Tips Easily with a Tip Ejector

An in-built, streamlined Tip Ejector enables easy tip ejection and comfortable access to bottles and tubes with narrow necks.

Work with a Good Grip

A specially designed large Grippy provides good grip and great ease of use while operating.

Assured Quality with ISO 8655 Conformed Calibration

The EZEEpette is calibrated in an ISO 17025 accredited laboratory according to ISO 8655 standards. A calibration certificate is included inside the product package. A calibration tool is also included for quick in-lab recalibration.

Work with a Comprehensive Volume Range

The EZEEpette Single Channel Variable Volume Micropipette is available in 6 unique volume ranges from 0.2 ?l to 1000 ?l.*Refer the table in technical specifications.

Autoclaving Parameters

The instrument is fully autoclavable at 121°C and 15 psi for a duration of 10-15 minutes.

Product Includes: Slide Carrier

In Simple Mode you just have to set your power supply to the desired parameters and press run. EV3xxx series has a firmware upgrade capability so future improvements and features will always be available.

Moreover EV3xxx series has a continuous logging combined with a real time clock so it’s possible to get an overview of previous runs, including possible down-times in case of mains power failures.

The complete EV series can keep it’s voltage constant at low currents without problem and will keep on functioning at low and high temperatures.

Features include:

• • On screen help in 4 languages to assist setting up the power supply parameters and solve errors.

• • Firmware updates allows for upgrades to the latest version via the USB interface. Feature requests can also be implemented via the firmware system.

• • Real Time Clock date and time are kept in a battery backup system and is used logging an electrophoresis run.

• • Various running modes:
    • • Simple Mode: just set voltage, current, power and time for a routine electrophoresis run.
    • • 9×9 Method Programming Mode: Up to 9 different programs, each with 9 steps, can be stored in the non-volatile memory. Each step is able to recall a next one, providing a flexible multiple step function for special techniques. Parameters of the running step can be changed temporarily without interrupting the run.
    • • Voltage Ramp Mode: a linear voltage gradient for any step provided the limiting current or power is not attained.
    • • Timer Mode: Timer or volt-hour controlled operation will automatically stop the run and sound an alarm.

• • Automatic cross-over Each model has constant voltage, constant current, constant power capabilities with automatic cross-over and shows which parameter is kept constant.

• • Automatic recovery after power failure

• • Password protection

• • Data-logging �?Data logging of about 100 hours of runs are automatically stored. Data includes data/time, voltage, current, power and date/time of following events: start, stop, pause, program number, step, changes, mains failure and auto restart.

• • Data Transfer �?Free data acquisition software for PC can be downloaded from our website. It allows to visualize and examine the stored run details.

• • Remote control �?EV3000 series can be controlled by a computer using special commands. These commands can be found in the support section of the Consort Website.
• Safety features:
  • • Ground leakage detection: protection from potential shock hazard when a ground leakage path is detected.
  • • Overload protection: full protection against any overload condition including accidental short circuit of the output.
  • • Smooth voltage rise: high voltage cannot suddenly appear at the outputs but will increase smoothly up to the pre-set limits.
  • • No load detection: prevents errors such as a bad or a dangling connection.
  • • Isolated communication: Optically isolated USB input/output connection to prevent any high voltage on the communication lines.

The  nanoPAC Mini Power supplies are an ultra compact and economic unit ideal for use with DNA (Horizontal) and Protein (Vertical) electrophoresis systems. With enhanced features, such as a maximum constant voltage up to 300 or 500V and maximum constant current output of 400mA the nanoPAC’s are capable of running all Cleaver Scientific horizontal multiSUB™ systems and vertical omniPAGE™ mini. The nanoPAC-500 is also capable of running the VS10W & VS20WAVE vertical units, as well as horizontal and vertical gel tanks from other manufacturers, These can be set on a continuous run or timed setting up to 999 minutes. The nanoPAC’s user- friendly interface is easily adjustable in 1V and 1mA increments, making it perfect for separations where precise settings are required. Its ultra compact size and two pairs of parallel power terminals, which can run two electrophoresis units simultaneously, save time and bench space.

VARIATIONS:

• 10 x Bromophenol Blue Loading Dye, 1ml
• 10 x Bromophenol Blue Loading Dye, 10ml
• Orange G Loading Dye, 1ml

Methylene blue solution is provided as a 10x concentrate.  To use, dilute 10x concentrate by mixing one part of methylene blue with nine parts of water (Eg. 100 ml of 10x concentrate added to 900 ml water).

The 1x methylene blue can then be used for staining of DNA bands within agarose gels. Place your Agarose gel into a small, clean gel-staining tray. Pour sufficient 1x methylene blue stain into a suitable tray in order to cover the agarose gel.  Allow to stand until DNA bands become visible within the agarose.  This can take up to 16 hours (overnight).  Once staining is complete, rinse the agarose gel with clean water.  Improved resolution of DNA bands can be achieved by allowing the gel to destain in a new clean trays and cover with clean water. For best results leave for approx 20 minutes on a shaker with a slow shaking motion. Changing the water during the time will also improve the destaining action.

Alternatively, DNA can be stained using 10x methylene blue concentrate.  Pour sufficient 10x methylene blue to cover the agarose gel and allow to stand for 5-10 minutes.  Once staining is complete, rinse the agarose gel in clean water.  Improved resolution of DNA bands can be achieved by allowing the gel to destain in clean water.

Once your gel has been stained and destained you can visualize your bands by placing the gel on a light table or if this is not available place the gel on a white surface.

Product Includes: 500 µl vial