B\u1ed1i c\u1ea3nh v\u1ec1 s\u1ef1 h\u1ecfng bia<\/a><\/li>
Danh m\u1ee5c x\u00e9t nghi\u1ec7m<\/a><\/li>
Ph\u00e1t tri\u1ec3n kh\u1ea3o nghi\u1ec7m<\/a><\/li>
X\u00e1c th\u1ef1c b\u00ean ngo\u00e0i<\/a>
- C\u00e1c th\u1eed nghi\u1ec7m kh\u00e1c trong Danh m\u1ee5c Bia<\/a><\/li><\/ul><\/li><\/ul><\/li>
- B\u1ea2N T\u00d3M T\u1eaeT<\/a><\/li><\/ul><\/nav><\/div>\n
  INVISIBLE SENTINEL<\/a> \u0111\u00e3 ph\u00e1t tri\u1ec3n m\u1ed9t c\u00f4ng ngh\u1ec7 ph\u00e2n t\u1eed m\u1edbi \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 VERIFLOW<\/a>, ki\u1ec3m so\u00e1t vi sinh v\u1eadt cho ng\u00e0nh bia b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p PCR, gi\u00fap c\u1ea3i thi\u1ec7n tri\u1ec7t \u0111\u1ec3 c\u00e1c v\u1ea5n \u0111\u1ec1 h\u1ea1n ch\u1ebf nh\u01b0 chi ph\u00ed cao, \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c th\u1ea5p. VERIFLOW<\/a> c\u0169ng \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf v\u1edbi m\u1ed9t b\u1ed9 t\u00ednh n\u0103ng to\u00e0n di\u1ec7n \u0111\u1ec3 \u0111\u00e1p \u1ee9ng nhu c\u1ea7u \u0111a d\u1ea1ng c\u1ee7a ng\u01b0\u1eddi d\u00f9ng cu\u1ed1i trong nhi\u1ec1u ng\u00e0nh c\u00f4ng nghi\u1ec7p. C\u00f4ng ngh\u1ec7 n\u00e0y s\u1eed d\u1ee5ng c\u00e1c nguy\u00ean t\u1eafc khoa h\u1ecdc \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ee9ng minh \u0111\u1ec3 ph\u00e1t hi\u1ec7n vi khu\u1ea9n k\u1ebft h\u1ee3p v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p khoa h\u1ecdc \u0111\u1ed9c quy\u1ec1n m\u1edbi \u0111\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c hi\u1ec7u su\u1ea5t t\u1ed1i \u01b0u. K\u1ebft qu\u1ea3 l\u00e0 m\u1ed9t n\u1ec1n t\u1ea3ng c\u00f4ng ngh\u1ec7 DNA Signature Capturing – m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p m\u1edbi \u0111\u1ec3 khu\u1ebfch \u0111\u1ea1i v\u00e0 nh\u1eadn d\u1ea1ng DNA k\u1ebft h\u1ee3p v\u1edbi quy tr\u00ecnh l\u00e0m vi\u1ec7c \u0111\u01a1n gi\u1ea3n, kh\u00f4ng b\u1ecb c\u1ea3n tr\u1edf t\u1eeb vi\u1ec7c thu th\u1eadp m\u1eabu \u0111\u1ebfn gi\u1ea3i th\u00edch d\u1eef li\u1ec7u. VERIFLOW<\/a> \u0111\u00e3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng tr\u00ean nhi\u1ec1u ng\u00e0nh c\u00f4ng nghi\u1ec7p bao g\u1ed3m an to\u00e0n th\u1ef1c ph\u1ea9m v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng \u0111\u1ed3 u\u1ed1ng, t\u1eadp trung v\u00e0o ng\u00e0nh s\u1ea3n xu\u1ea5t bia. K\u1ec3 t\u1eeb khi ra m\u1eaft BREWPAL\u00ae v\u00e0o th\u00e1ng 4 n\u0103m 2015, INVISIBLE SENTINEL<\/a> \u0111\u00e3 h\u1ee3p t\u00e1c v\u1edbi c\u00e1c nh\u00e0 m\u00e1y bia h\u00e0ng \u0111\u1ea7u tr\u00ean to\u00e0n th\u1ebf gi\u1edbi \u0111\u1ec3 gi\u1ea3i quy\u1ebft c\u00e1c m\u1ed1i lo ng\u1ea1i v\u1ec1 ch\u1ea5t l\u01b0\u1ee3ng vi sinh v\u1eadt c\u1ee7a h\u1ecd. H\u00e0ng tr\u0103m nh\u00e0 m\u00e1y bia t\u1ea1i h\u01a1n 15 qu\u1ed1c gia hi\u1ec7n \u0111ang s\u1eed d\u1ee5ng c\u00f4ng ngh\u1ec7 VERIFLOW<\/a> \u0111\u1ec3 b\u1ea3o v\u1ec7 th\u01b0\u01a1ng hi\u1ec7u c\u1ee7a m\u00ecnh v\u00e0 \u0111\u1ea3m b\u1ea3o bia \u0111\u00e1p \u1ee9ng c\u00e1c ti\u00eau chu\u1ea9n ch\u1ea5t l\u01b0\u1ee3ng nghi\u00eam ng\u1eb7t nh\u1ea5t.<\/p>\n
  <\/span>T\u1ed4NG QUAN V\u1ec0 C\u00d4NG NGH\u1ec6 VERIFLOW<\/a><\/strong><\/span><\/h2>\n
  K\u1ebft h\u1ee3p c\u00e1c ph\u01b0\u01a1ng ph\u00e1p khoa h\u1ecdc m\u1edbi, VERIFLOW\u00ae<\/a> k\u1ebft h\u1ee3p c\u00f4ng ngh\u1ec7 PCR \u0111i\u1ec3m cu\u1ed1i m\u1ea1nh m\u1ebd v\u1edbi kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n d\u1ef1a tr\u00ean d\u00f2ng ch\u1ea3y bia.<\/p>\n
  Qu\u00e1 tr\u00ecnh n\u00e0y b\u1eaft \u0111\u1ea7u b\u1eb1ng vi\u1ec7c khu\u1ebfch \u0111\u1ea1i DNA m\u1ee5c ti\u00eau, m\u1ed9t b\u01b0\u1edbc thi\u1ebft y\u1ebfu trong ch\u1ea9n \u0111o\u00e1n ph\u00e2n t\u1eed d\u1ef1a tr\u00ean PCR. Khu\u1ebfch \u0111\u1ea1i l\u00e0 qu\u00e1 tr\u00ecnh t\u0103ng s\u1ed1 l\u01b0\u1ee3ng b\u1ea3n sao c\u1ee7a m\u1ed9t chu\u1ed7i DNA c\u1ee5 th\u1ec3 \u0111\u1ebfn m\u1ee9c c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c. C\u00f3 m\u1ed9t s\u1ed1 c\u00f4ng ngh\u1ec7 khu\u1ebfch \u0111\u1ea1i kh\u00e1c nhau, tuy nhi\u00ean VERIFLOW<\/a> s\u1eed d\u1ee5ng c\u00e1ch ti\u1ebfp c\u1eadn \u0111\u1ed9c quy\u1ec1n, \u0111\u1ed9c \u0111\u00e1o. C\u00e1c enzyme \u0111\u1ed9c quy\u1ec1n \u0111\u01b0\u1ee3c VERIFLOW<\/a> s\u1eed d\u1ee5ng trong qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf m\u1ea1nh m\u1ebd v\u00e0 c\u00f3 kh\u1ea3 n\u0103ng ch\u1ed1ng l\u1ea1i s\u1ef1 \u1ee9c ch\u1ebf. \u0110i\u1ec1u n\u00e0y cho ph\u00e9p qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i di\u1ec5n ra m\u00e0 kh\u00f4ng b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi c\u00e1c ch\u1ea5t \u1ee9c ch\u1ebf hi\u1ec7n di\u1ec7n t\u1ef1 nhi\u00ean trong nhi\u1ec1u m\u1eabu kh\u00e1c nhau. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p truy\u1ec1n th\u1ed1ng y\u00eau c\u1ea7u tinh ch\u1ebf m\u1eabu nghi\u00eam ng\u1eb7t \u0111\u1ec3 lo\u1ea1i b\u1ecf c\u00e1c ch\u1ea5t \u1ee9c ch\u1ebf v\u00e0 \u0111\u1ea3m b\u1ea3o khu\u1ebfch \u0111\u1ea1i th\u00e0nh c\u00f4ng. C\u00f4ng ngh\u1ec7 c\u00f4 l\u1eadp enzyme v\u00e0 ch\u1ea5t \u1ee9c ch\u1ebf \u0111\u1ed9c quy\u1ec1n c\u1ee7a VERIFLOW<\/a> lo\u1ea1i b\u1ecf nhu c\u1ea7u tinh ch\u1ebf m\u1eabu ho\u1eb7c t\u00e1ch chi\u1ebft DNA, nh\u1edd \u0111\u00f3 gi\u1ea3m \u0111\u00e1ng k\u1ec3 \u0111\u1ed9 ph\u1ee9c t\u1ea1p c\u1ee7a quy tr\u00ecnh x\u00e9t nghi\u1ec7m t\u1ed5ng th\u1ec3. S\u1ef1 c\u1ea3i thi\u1ec7n v\u1ec1 quy tr\u00ecnh l\u00e0m vi\u1ec7c v\u00e0 t\u00ednh m\u1ea1nh m\u1ebd n\u00e0y cho ph\u00e9p m\u1ed9t s\u1ed1 l\u1ee3i \u00edch s\u1ebd \u0111\u01b0\u1ee3c th\u1ea3o lu\u1eadn s\u00e2u h\u01a1n trong b\u00e0i vi\u1ebft n\u00e0y.<\/p>\n
  $\"\"$ <\/p>\n
  B\u01b0\u1edbc ti\u1ebfp theo trong quy tr\u00ecnh ph\u00e1t hi\u1ec7n l\u00e0 ph\u00e1t hi\u1ec7n DNA m\u1ee5c ti\u00eau \u0111\u01b0\u1ee3c khu\u1ebfch \u0111\u1ea1i t\u1eeb m\u1eabu m\u1ed9t c\u00e1ch h\u1ee3p l\u00fd. Kh\u00f4ng gi\u1ed1ng nh\u01b0 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p d\u1ef1a tr\u00ean PCR th\u1eddi gian th\u1ef1c (RT) truy\u1ec1n th\u1ed1ng, VERIFLOW<\/a> kh\u00f4ng s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n quang h\u1ecdc DNA khu\u1ebfch \u0111\u1ea1i. Ph\u00e1t hi\u1ec7n quang h\u1ecdc c\u00f3 m\u1ed9t s\u1ed1 nh\u01b0\u1ee3c \u0111i\u1ec3m bao g\u1ed3m y\u00eau c\u1ea7u \u201cl\u00e0m s\u1ea1ch\u201d m\u1eabu \u0111\u1ec3 tr\u00e1nh nhi\u1ec5u v\u1edbi c\u1ea3m bi\u1ebfn quang. Thay v\u00e0o \u0111\u00f3, vi\u1ec7c s\u1eed d\u1ee5ng h\u1ec7 th\u1ed1ng ph\u00e1t hi\u1ec7n d\u1ea5u hi\u1ec7u DNA duy nh\u1ea5t cho ph\u00e9p VERIFLOW<\/a> thu th\u1eadp DNA khu\u1ebfch \u0111\u1ea1i tr\u00ean m\u1ed9t cassette c\u1ea7m tay. DNA m\u1ee5c ti\u00eau li\u00ean k\u1ebft v\u1edbi v\u1ea1ch th\u1eed nghi\u1ec7m tr\u00ean cassette \u0111\u1ec3 cho ph\u00e9p gi\u1ea3i th\u00edch k\u1ebft qu\u1ea3 d\u1ec5 d\u00e0ng.<\/p>\n
  Ti\u1ebfn b\u1ed9 quan tr\u1ecdng cu\u1ed1i c\u00f9ng trong qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n VERIFLOW<\/a> l\u00e0 gi\u1ea3i quy\u1ebft kh\u1ea3 n\u0103ng DNA di chuy\u1ec3n qua m\u1ed9t t\u1eadp h\u1ee3p mao m\u1ea1ch \u0111\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n cu\u1ed1i c\u00f9ng v\u00e0 hi\u1ec3n th\u1ecb k\u1ebft qu\u1ea3 trong m\u1ed9t cassette c\u1ea7m tay. VERIFLOW<\/a> \u0111\u00e3 ph\u00e1t tri\u1ec3n m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ti\u1ebfp c\u1eadn d\u00f2ng ch\u1ea3y th\u1eb3ng \u0111\u1ee9ng m\u1ea1nh m\u1ebd v\u00e0 kh\u1ea3 thi. C\u00e1ch ti\u1ebfp c\u1eadn n\u00e0y, k\u1ebft h\u1ee3p v\u1edbi nh\u1eefng s\u1eeda \u0111\u1ed5i v\u1ec1 c\u1ea3 v\u1eadt l\u00fd v\u00e0 v\u1eadt li\u1ec7u \u0111i\u1ec1u h\u00f2a d\u00f2ng ch\u1ea3y, cho ph\u00e9p DNA nguy\u00ean v\u1eb9n \u0111i \u0111\u1ebfn v\u1ea1ch th\u1eed nghi\u1ec7m cu\u1ed1i c\u00f9ng m\u1ed9t c\u00e1ch th\u00e0nh c\u00f4ng. T\u00ednh n\u0103ng n\u00e0y c\u1ee7a c\u00f4ng ngh\u1ec7 d\u00f2ng ch\u1ea3y d\u1ecdc cho k\u1ebft qu\u1ea3 t\u1ee9c th\u00ec v\u00e0 gi\u1ea3m \u0111\u00e1ng k\u1ec3 d\u00f2ng ch\u1ea3y ng\u01b0\u1ee3c, hai h\u1ea1n ch\u1ebf li\u00ean quan \u0111\u1ebfn c\u00f4ng ngh\u1ec7 d\u00f2ng ch\u1ea3y ngang, m\u1ed9t lo\u1ea1i c\u00f4ng ngh\u1ec7 ch\u1ea9n \u0111o\u00e1n nhanh thay th\u1ebf. K\u1ebft qu\u1ea3 VERIFLOW<\/a> l\u00e0 k\u1ebft qu\u1ea3 \u0111\u1ecbnh t\u00ednh v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng \u0110\u1ea7u \u0111\u1ecdc quang h\u1ecdc cho ph\u00e9p ph\u00e1t hi\u1ec7n v\u00e0 li\u1ec7t k\u00ea \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 r\u1ee7i ro vi sinh v\u1eadt.<\/p>\n
  <\/span>\u00c1P D\u1ee4NG KHOA H\u1eccC V\u00c0O C\u00d4NG NGH\u1ec6<\/strong><\/span><\/h2>\n<\/span>Ph\u01b0\u01a1ng ph\u00e1p kh\u00f4ng c\u1ea7n chi\u1ebft xu\u1ea5t DNA<\/strong><\/span><\/h3>\nTr\u01b0\u1edbc \u0111\u00e2y, c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u1eed \u0111\u00e3 c\u1ea3i thi\u1ec7n \u0111\u00e1ng k\u1ec3 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9 nh\u1ea1y nh\u01b0ng chi ph\u00ed cao v\u00e0 khi th\u1ef1c hi\u1ec7n c\u1ea7n y\u00eau c\u1ea7u thi\u1ebft b\u1ecb chuy\u00ean d\u1ee5ng c\u0169ng nh\u01b0 nh\u00e2n vi\u00ean \u0111\u01b0\u1ee3c \u0111\u00e0o t\u1ea1o chuy\u00ean s\u00e2u. M\u1ed9t s\u1ed1 y\u1ebfu t\u1ed1 g\u00f3p ph\u1ea7n d\u1eabn \u0111\u1ebfn chi ph\u00ed cao l\u00e0 nhu c\u1ea7u chu\u1ea9n b\u1ecb m\u1eabu ph\u1ee9c t\u1ea1p tr\u01b0\u1edbc khi khu\u1ebfch \u0111\u1ea1i PCR, sau \u0111\u00f3 l\u00e0 ph\u00e1t hi\u1ec7n quang h\u1ecdc c\u00e1c s\u1ea3n ph\u1ea9m b\u1eb1ng thi\u1ebft b\u1ecb chi ph\u00ed cao . Ph\u01b0\u01a1ng ph\u00e1p d\u1ef1a tr\u00ean RT-PCR \u0111\u00f2i h\u1ecfi c\u00e1c b\u01b0\u1edbc t\u00e1ch chi\u1ebft DNA ph\u1ee9c t\u1ea1p do c\u00f3 ch\u1ea5t \u1ee9c ch\u1ebf trong bia. S\u1ef1 \u1ee9c ch\u1ebf PCR c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c g\u00e2y ra b\u1edfi s\u1ef1 b\u1ea5t ho\u1ea1t c\u1ee7a enzyme v\u00e0\/ho\u1eb7c c\u00e1c t\u01b0\u01a1ng t\u00e1c kh\u00f4ng \u0111\u1eb7c hi\u1ec7u gi\u1eefa m\u1ed3i v\u00e0 DNA m\u1ee5c ti\u00eau. Trong c\u00e1c ph\u01b0\u01a1ng ph\u00e1p n\u00e0y, vi\u1ec7c kh\u00f4ng s\u1eed d\u1ee5ng m\u1eabu DNA c\u00f3 \u0111\u1ed9 tinh khi\u1ebft cao c\u00f3 th\u1ec3 l\u00e0m t\u0103ng kh\u1ea3 n\u0103ng x\u1ea3y ra k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh gi\u1ea3 v\u00e0 \u00e2m t\u00ednh gi\u1ea3. VERIFLOW\u00ae <\/a>\u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3 \u0111\u00e1p \u1ee9ng nh\u1eefng th\u00e1ch th\u1ee9c n\u00e0y. C\u00f4ng ngh\u1ec7 n\u00e0y s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p kh\u00f4ng c\u1ea7n chi\u1ebft DNA gi\u00fap gi\u1ea3m \u0111\u00e1ng k\u1ec3 th\u1eddi gian chuy\u1ec3n m\u1eabu t\u1eeb t\u00fai t\u0103ng sinh sang \u1ed1ng PCR. C\u00f4ng ngh\u1ec7 \u0111\u1ec7m m\u1ea1nh m\u1ebd k\u1ebft h\u1ee3p v\u1edbi DNA polymerase kh\u00e1ng ch\u1ea5t \u1ee9c ch\u1ebf kh\u00f4ng ch\u1ec9 mang l\u1ea1i \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh v\u00e0 \u0111\u1ed9 nh\u1ea1y v\u01b0\u1ee3t tr\u1ed9i m\u00e0 c\u00f2n cho ph\u00e9p khu\u1ebfch \u0111\u1ea1i hi\u1ec7u qu\u1ea3 c\u00e1c m\u1ee5c ti\u00eau DNA tr\u1ef1c ti\u1ebfp t\u1eeb c\u00e1c m\u1eabu th\u1ef1c ph\u1ea9m th\u00f4, do \u0111\u00f3 lo\u1ea1i b\u1ecf nhu c\u1ea7u tinh ch\u1ebf DNA. Hi\u1ec7u su\u1ea5t c\u1ee7a c\u00e1c bi\u1ebfn th\u1ec3 enzyme n\u00e0y \u0111\u01b0\u1ee3c n\u00e2ng cao h\u01a1n n\u1eefa b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c chi\u1ebfn l\u01b0\u1ee3c x\u1eed l\u00fd m\u1eabu m\u1edbi gi\u00fap \u0111\u01a1n gi\u1ea3n h\u00f3a, \u0111\u1ea9y nhanh v\u00e0 gi\u1ea3m chi ph\u00ed so v\u1edbi c\u00e1c x\u00e9t nghi\u1ec7m d\u1ef1a tr\u00ean PCR ph\u1ee9c t\u1ea1p. Vi\u1ec7c lo\u1ea1i b\u1ecf qu\u00e1 tr\u00ecnh tinh ch\u1ebf m\u1eabu gi\u00fap gi\u1ea3m \u0111\u00e1ng k\u1ec3 nhi\u1ec1u thao t\u00e1c m\u1eabu, do \u0111\u00f3 gi\u1ea3m nguy c\u01a1 l\u1ed7i do ng\u01b0\u1eddi d\u00f9ng. Ph\u1ea3n \u1ee9ng PCR VERIFLOW \u0111\u01b0\u1ee3c b\u1ea3o v\u1ec7 h\u01a1n n\u1eefa kh\u1ecfi kh\u1ea3 n\u0103ng l\u00e2y nhi\u1ec5m b\u1eb1ng c\u00e1ch thay th\u1ebf dTTP (deoxythymidine triphosphate) b\u1eb1ng dUTP (deoxyuridine triphosphate) trong h\u1ed7n h\u1ee3p dNTP (deoxynucleotide) c\u00f9ng v\u1edbi Uracil-DNA Glycosylase (UDG). Tr\u01b0\u1edbc khi b\u1eaft \u0111\u1ea7u PCR, ho\u1ea1t \u0111\u1ed9ng UDG s\u1ebd t\u00e1ch baz\u01a1 uracil trong DNA ch\u1ee9a dUTP (khu\u1ebfch \u0111\u1ea1i) ng\u0103n ch\u1eb7n qu\u00e1 tr\u00ecnh t\u00e1i khu\u1ebfch \u0111\u1ea1i do nhi\u1ec5m b\u1ea9n.<\/p>\n
  <\/span>Khu\u1ebfch \u0111\u1ea1i DNA<\/strong><\/span><\/h3>\nPCR l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt t\u1ea1i ch\u1ed7 cho ph\u00e9p nh\u00e2n m\u1ed9t chu\u1ed7i DNA c\u1ee5 th\u1ec3 trong m\u1ed9t m\u1eabu. Do \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao, PCR \u0111\u00e3 tr\u1edf th\u00e0nh m\u1ed9t k\u1ef9 thu\u1eadt ph\u1ed5 bi\u1ebfn trong l\u0129nh v\u1ef1c c\u00f4ng ngh\u1ec7 sinh h\u1ecdc v\u00e0 ch\u1ea9n \u0111o\u00e1n. Qu\u00e1 tr\u00ecnh n\u00e0y s\u1eed d\u1ee5ng c\u00e1c oligonucleotide b\u1ed5 sung (m\u1ed3i) \u0111\u1ec3 nh\u1eadn bi\u1ebft c\u1ee5 th\u1ec3 tr\u00ecnh t\u1ef1 di truy\u1ec1n duy nh\u1ea5t trong b\u1ed9 gen c\u1ee7a m\u1ea7m b\u1ec7nh m\u1ee5c ti\u00eau. \u0110o\u1ea1n DNA c\u1ee5 th\u1ec3 n\u00e0y tr\u1ea3i qua qu\u00e1 tr\u00ecnh l\u00e0m n\u00f3ng v\u00e0 l\u00e0m m\u00e1t ba b\u01b0\u1edbc theo chu k\u1ef3, c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 chu tr\u00ecnh nhi\u1ec7t, trong \u1ed1ng thu\u1ed1c th\u1eed PCR ch\u1ee9a h\u1ed7n h\u1ee3p t\u1ed5ng th\u1ec3 g\u1ed3m DNA polymerase, nucleotide v\u00e0 m\u1ed3i. \u0110\u1ed1i v\u1edbi d\u00f2ng th\u1eed nghi\u1ec7m VERIFLOW<\/a>, quy tr\u00ecnh n\u00e0y \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong M\u00e1y \u0111i\u1ec1u nhi\u1ec7t VERIPRO.<\/p>\n
  <\/span>Ba chu k\u1ef3 PCR:<\/strong><\/span><\/h3>\n
  <\/span>Bi\u1ebfn t\u00ednh<\/strong><\/span><\/h4>\n
  DNA m\u1ee5c ti\u00eau s\u1ee3i \u0111\u00f4i \u0111\u01b0\u1ee3c bi\u1ebfn t\u00ednh \u1edf nhi\u1ec7t \u0111\u1ed9 cao th\u00e0nh hai s\u1ee3i \u0111\u01a1n ri\u00eang bi\u1ec7t<\/p>\n
  <\/span>\u1ee6 l\u1edbp l\u00f3t<\/strong><\/span><\/h4>\n
  Nhi\u1ec7t \u0111\u1ed9 \u0111\u01b0\u1ee3c h\u1ea1 xu\u1ed1ng \u0111\u1ec3 cho ph\u00e9p hai oligonucleotide t\u1ed5ng h\u1ee3p (ho\u1eb7c m\u1ed3i) \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u tr\u01b0\u1edbc lai v\u1edbi DNA m\u1ee5c ti\u00eau.<\/p>\n
  <\/span>Ph\u1ea7n m\u1edf r\u1ed9ng<\/strong><\/span><\/h4>\n
  Nhi\u1ec7t \u0111\u1ed9 \u0111\u01b0\u1ee3c t\u0103ng l\u00ean \u0111\u1ec3 k\u00edch ho\u1ea1t qu\u00e1 tr\u00ecnh tr\u00f9ng h\u1ee3p, trong \u0111\u00f3 ph\u00e2n t\u1eed DNA s\u1ee3i \u0111\u00f4i m\u1edbi \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u (khu\u1ebfch \u0111\u1ea1i) \u0111\u01b0\u1ee3c t\u1ea1o ra b\u1eb1ng c\u00e1ch m\u1edf r\u1ed9ng c\u00e1c \u0111o\u1ea1n m\u1ed3i b\u1ed5 sung cho m\u1eabu DNA s\u1ee3i \u0111\u01a1n<\/p>\n
  Ba b\u01b0\u1edbc n\u00e0y \u0111\u01b0\u1ee3c l\u1eb7p l\u1ea1i h\u01a1n 30 l\u1ea7n (t\u00f9y thu\u1ed9c v\u00e0o m\u1ee5c ti\u00eau quan t\u00e2m), d\u1eabn \u0111\u1ebfn s\u1ef1 khu\u1ebfch \u0111\u1ea1i theo c\u1ea5p s\u1ed1 nh\u00e2n c\u1ee7a DNA m\u1ee5c ti\u00eau<\/p>\n
  $\"\"$ <\/p>\n
  <\/span>Tr\u1ef1c quan h\u00f3a k\u1ebft qu\u1ea3<\/strong><\/span><\/h4>\n
  M\u1ed9t trong nh\u1eefng y\u1ebfu t\u1ed1 th\u00e1ch th\u1ee9c nh\u1ea5t trong vi\u1ec7c \u0111\u00e1p \u1ee9ng nhu c\u1ea7u th\u1eed nghi\u1ec7m ph\u00e2n t\u1eed c\u1ee7a ng\u00e0nh bia l\u00e0 quy tr\u00ecnh c\u1ea7n thi\u1ebft \u0111\u1ec3 \u0111\u01a1n gi\u1ea3n h\u00f3a vi\u1ec7c di\u1ec5n gi\u1ea3i d\u1eef li\u1ec7u. Vi\u1ec7c s\u1eed d\u1ee5ng gi\u1ea3i th\u00edch d\u1eef li\u1ec7u ho\u00e0n to\u00e0n d\u1ef1a tr\u00ean ph\u1ea7n m\u1ec1m l\u00e0m t\u0103ng chi ph\u00ed thi\u1ebft b\u1ecb v\u00e0 \u0111\u1ed9 ph\u1ee9c t\u1ea1p c\u1ee7a quy tr\u00ecnh l\u00e0m vi\u1ec7c. Ph\u00ed b\u1ea3o tr\u00ec h\u00e0ng n\u0103m v\u00e0 h\u1ee3p \u0111\u1ed3ng d\u1ecbch v\u1ee5 c\u0169ng l\u00e0m t\u0103ng th\u00eam chi ph\u00ed thi\u1ebft b\u1ecb v\u1ed1n \u0111\u00e3 cao.<\/p>\n
  VERIFLOW\u00ae<\/a> l\u00e0 duy nh\u1ea5t. Vi\u1ec7c hi\u1ec3n th\u1ecb k\u1ebft qu\u1ea3 trong VERIFLOW<\/a> d\u1ef1a tr\u00ean c\u00f4ng ngh\u1ec7 d\u00f2ng ch\u1ea3y \u0111\u01b0\u1ee3c \u0111\u1eb7t b\u00ean trong m\u1ed9t cassette c\u1ea7m tay. Sau khi \u0111\u01b0a m\u1eabu v\u00e0o, li\u00ean h\u1ee3p protein-v\u00e0ng d\u1ea1ng keo \u0111\u01b0\u1ee3c kh\u1eed n\u01b0\u1edbc v\u00e0 ph\u1ea3n \u1ee9ng v\u1edbi khu\u1ebfch \u0111\u1ea1i \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u trong m\u1eabu. H\u1ed7n h\u1ee3p n\u00e0y ch\u1ea3y d\u1ecdc m\u00e0ng nitrocellulose \u0111\u01b0\u1ee3c c\u1ed1 \u0111\u1ecbnh b\u1eb1ng kh\u00e1ng th\u1ec3 b\u1eaft gi\u1eef tr\u00ean v\u1ea1ch th\u1eed. B\u1ed9 khu\u1ebfch \u0111\u1ea1i, \u0111\u01b0\u1ee3c k\u1eb9p gi\u1eefa kh\u00e1ng th\u1ec3 li\u00ean h\u1ee3p protein-v\u00e0ng v\u00e0 kh\u00e1ng th\u1ec3 b\u1eaft gi\u1eef, t\u1eadp h\u1ee3p l\u1ea1i tr\u00ean v\u1ea1ch th\u1eed nghi\u1ec7m \u0111\u1ec3 t\u1ea1o ra v\u1ea1ch m\u00e0u \u0111\u1ecf th\u1eabm c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c b\u1eb1ng m\u1eaft th\u01b0\u1eddng cho th\u1ea5y s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a \u00f4 nhi\u1ec5m ch\u1ec9 trong v\u00f2ng 3 ph\u00fat sau khi \u0111\u01b0a m\u1eabu v\u00e0o. M\u00e0ng n\u00e0y c\u0169ng ch\u1ee9a m\u1ed9t v\u1ea1ch ki\u1ec3m so\u00e1t, sao cho m\u1ed9t v\u1ea1ch duy nh\u1ea5t bi\u1ec3u th\u1ecb k\u1ebft qu\u1ea3 \u00e2m t\u00ednh v\u00e0 hai v\u1ea1ch bi\u1ec3u th\u1ecb k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh.<\/p>\n
  \u0110\u1ec3 thu \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 \u0111\u1ecbnh l\u01b0\u1ee3ng, c\u01b0\u1eddng \u0111\u1ed9 v\u1ea1ch th\u1eed c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u0111o b\u1eb1ng \u0110\u1ea7u \u0111\u1ecdc VERIFLOW<\/a>. Cassette ph\u00f9 h\u1ee3p v\u1edbi gi\u00e1 \u0111\u1ee1 c\u1ee7a \u0111\u1ea7u \u0111\u1ecdc \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 t\u1eebng Cassette ri\u00eang l\u1ebb. M\u1ed9t trong nh\u1eefng y\u1ebfu t\u1ed1 th\u00e1ch th\u1ee9c nh\u1ea5t trong vi\u1ec7c \u0111\u00e1p \u1ee9ng nhu c\u1ea7u th\u1eed nghi\u1ec7m ph\u00e2n t\u1eed c\u1ee7a ng\u00e0nh bia l\u00e0 quy tr\u00ecnh c\u1ea7n thi\u1ebft \u0111\u1ec3 \u0111\u01a1n gi\u1ea3n h\u00f3a vi\u1ec7c di\u1ec5n gi\u1ea3i d\u1eef li\u1ec7u. Vi\u1ec7c s\u1eed d\u1ee5ng gi\u1ea3i th\u00edch d\u1eef li\u1ec7u ho\u00e0n to\u00e0n d\u1ef1a tr\u00ean ph\u1ea7n m\u1ec1m l\u00e0m t\u0103ng chi ph\u00ed thi\u1ebft b\u1ecb v\u00e0 \u0111\u1ed9 ph\u1ee9c t\u1ea1p c\u1ee7a quy tr\u00ecnh l\u00e0m vi\u1ec7c. Ph\u00ed b\u1ea3o tr\u00ec h\u00e0ng n\u0103m v\u00e0 h\u1ee3p \u0111\u1ed3ng d\u1ecbch v\u1ee5 c\u0169ng l\u00e0m t\u0103ng th\u00eam chi ph\u00ed thi\u1ebft b\u1ecb v\u1ed1n \u0111\u00e3 cao. \u0110\u1ea7u \u0111\u1ecdc \u0111\u01b0\u1ee3c l\u1eadp tr\u00ecnh v\u1edbi m\u1ed9t \u0111\u01b0\u1eddng cong hi\u1ec7u chu\u1ea9n \u0111\u1ec3 \u0111o t\u1ef7 l\u1ec7 c\u1ee7a \u0111\u01b0\u1eddng th\u1eed v\u1edbi c\u01b0\u1eddng \u0111\u1ed9 c\u1ee7a \u0111\u01b0\u1eddng \u0111i\u1ec1u khi\u1ec3n v\u00e0 t\u01b0\u01a1ng quan gi\u00e1 tr\u1ecb \u0111\u00f3 v\u1edbi \u0111\u01b0\u1eddng cong hi\u1ec7u chu\u1ea9n. S\u1eed d\u1ee5ng thu\u1eadt to\u00e1n t\u00edch h\u1ee3p n\u00e0y, \u0111\u1ea7u \u0111\u1ecdc hi\u1ec3n th\u1ecb k\u1ebft qu\u1ea3 \u0111\u1ecbnh l\u01b0\u1ee3ng (t\u1ebf b\u00e0o\/mL) t\u1eeb d\u1ea3i th\u1eed nghi\u1ec7m, t\u1ea1o ra con s\u1ed1 ch\u00ednh x\u00e1c li\u00ean quan \u0111\u1ebfn c\u01b0\u1eddng \u0111\u1ed9 v\u1ea1ch th\u1eed nghi\u1ec7m.<\/p>\n
  <\/span>PH\u00c1T TRI\u1ec2N V\u00c0 X\u00c1C TH\u1ef0C DANH M\u1ee4C BIA<\/strong><\/span><\/h2>\n
  <\/span>B\u1ed1i c\u1ea3nh v\u1ec1 s\u1ef1 h\u1ecfng bia<\/strong><\/span><\/h3>\n
  Bia l\u00e0 \u0111\u1ed3 u\u1ed1ng c\u00f3 \u0111\u1ed9 pH th\u1ea5p (3,8 \u2013 4,7), ch\u1ee9a c\u1ed3n (0,5 \u2013 10% w\/v) \u0111\u01b0\u1ee3c c\u00f4ng nh\u1eadn l\u00e0 s\u1ea3n ph\u1ea9m \u1ed5n \u0111\u1ecbnh v\u1ec1 m\u1eb7t vi sinh. Vi\u1ec7c thi\u1ebfu ch\u1ea5t dinh d\u01b0\u1ee1ng v\u00e0 oxy (< 0,1 ppm), c\u00f9ng v\u1edbi h\u00e0m l\u01b0\u1ee3ng carbon dioxide cao (0\/5 w\/w) v\u00e0 t\u00e1c d\u1ee5ng kh\u00e1ng khu\u1ea9n c\u1ee7a axit \u0111\u1eafng hoa bia (17 \u2013 55 ppm axit iso-\u03b1), t\u1ea1o ra m\u00f4i tr\u01b0\u1eddng kh\u00f4ng thu\u1eadn l\u1ee3i. m\u00f4i tr\u01b0\u1eddng cho vi sinh v\u1eadt h\u01b0 h\u1ecfng t\u1ed3n t\u1ea1i v\u00e0 ph\u00e1t tri\u1ec3n. M\u1eb7c d\u00f9 c\u00e1c \u0111i\u1ec1u ki\u1ec7n d\u01b0\u1eddng nh\u01b0 kh\u00f4ng t\u1ed1i \u01b0u, c\u00e1c vi sinh v\u1eadt g\u00e2y h\u01b0 h\u1ecfng bia v\u1eabn v\u00f4 t\u00ecnh x\u00e2m nh\u1eadp v\u00e0o b\u00f9n men, b\u1ec3 l\u00ean men, b\u1ec3 ch\u1ee9a v\u00e0 s\u1ea3n ph\u1ea9m \u0111\u00f3ng g\u00f3i. K\u1ebft qu\u1ea3 l\u00e0 \u0111\u1ed9 \u0111\u1ee5c t\u0103ng l\u00ean v\u00e0 nh\u1eefng m\u00f9i v\u1ecb kh\u00f3 ch\u1ecbu trong bia. M\u1eb7c d\u00f9 \u0111\u00f3ng g\u00f3i v\u00f4 tr\u00f9ng \u0111\u1ed1i v\u1edbi bia \u0111\u00f3ng h\u1ed9p v\u00e0 \u0111\u00f3ng chai, \u00f4 nhi\u1ec5m sinh h\u1ecdc c\u00f3 th\u1ec3 x\u1ea3y ra sau \u0111\u00f3ng g\u00f3i do qu\u00e1 tr\u00ecnh thanh tr\u00f9ng ho\u1eb7c l\u1ecdc kh\u00f4ng hi\u1ec7u qu\u1ea3 d\u1eabn \u0111\u1ebfn \u1ea3nh h\u01b0\u1edfng x\u1ea5u \u0111\u1ebfn s\u1ea3n ph\u1ea9m \u0111\u01b0\u1ee3c \u0111\u00f3ng g\u00f3i. Bia h\u01b0 h\u1ecfng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn ch\u1ea5t l\u01b0\u1ee3ng s\u1ea3n ph\u1ea9m v\u00e0 c\u00f3 th\u1ec3 g\u00e2y thi\u1ec7t h\u1ea1i kinh t\u1ebf nghi\u00eam tr\u1ecdng cho nh\u00e0 m\u00e1y bia.<\/p>\n
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  B\u1ea3ng 1:<\/strong> Danh m\u1ee5c s\u1ea3n ph\u1ea9m bia<\/figcaption><\/figure>\n<\/span>Danh m\u1ee5c x\u00e9t nghi\u1ec7m<\/strong><\/span><\/h3>\nINVISIBLE SENTINEL<\/a> b\u1eaft \u0111\u1ea7u ph\u00e1t tri\u1ec3n danh m\u1ee5c s\u1ea3n ph\u1ea9m, \u0111\u01b0\u1ee3c h\u1ed7 tr\u1ee3 b\u1eb1ng c\u00e1ch nh\u1eafm m\u1ee5c ti\u00eau v\u00e0o 6 danh m\u1ee5c s\u1ea3n ph\u1ea9m l\u00e0m h\u1ecfng bia h\u00e0ng \u0111\u1ea7u (B\u1ea3ng 1).<\/strong><\/p>\n
  M\u1ed7i x\u00e9t nghi\u1ec7m trong danh m\u1ee5c \u0111\u1ec1u \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n c\u00f3 m\u1ee5c \u0111\u00edch v\u00e0 \u0111\u01b0\u1ee3c x\u00e1c nh\u1eadn r\u1ed9ng r\u00e3i \u0111\u1ec3 \u0111\u00e1p \u1ee9ng c\u00e1c ti\u00eau chu\u1ea9n cao nh\u1ea5t v\u1ec1 t\u00ednh to\u00e0n di\u1ec7n, t\u00ednh \u0111\u1ed9c quy\u1ec1n v\u00e0 \u0111\u1ed9 b\u1ec1n t\u1ed5ng th\u1ec3. B\u00e0i vi\u1ebft n\u00e0y s\u1ebd tr\u00ecnh b\u00e0y chi ti\u1ebft v\u1ec1 qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n v\u00e0 x\u00e1c nh\u1eadn th\u1eed nghi\u1ec7m \u0111\u1ea7u ti\u00ean v\u00e0 th\u1eed nghi\u1ec7m d\u1eabn \u0111\u1ea7u, BREWPAL. T\u1ea5t c\u1ea3 c\u00e1c x\u00e9t nghi\u1ec7m \u0111\u1ec1u tu\u00e2n theo c\u00f9ng m\u1ed9t quy tr\u00ecnh ch\u1ea5t l\u01b0\u1ee3ng cao \u0111\u1ec3 ph\u00e1t tri\u1ec3n v\u00e0 x\u00e1c nh\u1eadn v\u00e0 \u0111\u01b0\u1ee3c t\u00f3m t\u1eaft d\u01b0\u1edbi \u0111\u00e2y<\/p>\n
  $\"\"$ <\/p>\n
  <\/span>Ph\u00e1t tri\u1ec3n kh\u1ea3o nghi\u1ec7m<\/strong><\/span><\/h3>\n
  Vi khu\u1ea9n axit lactic (LAB) thu\u1ed9c h\u1ecd Lactobacillus BREWLAP\u00ae Vi khu\u1ea9n s\u1ea3n xu\u1ea5t axit lactic v\u00e0 chi Pediococcus g\u00e2y ra kho\u1ea3ng 60 \u2013 70% c\u00e1c v\u1ee5 h\u01b0 h\u1ecfng bia. Hai lo\u1ea1i protein kh\u00e1ng hoa bia \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 horA v\u00e0 horC, \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 h\u1ee3p ch\u1ea5t g\u00e2y h\u01b0 h\u1ecfng g\u00e2y ra hi\u1ec7n t\u01b0\u1ee3ng \u0111\u1ee5c v\u00e0 x\u1ed1p, d\u1eabn \u0111\u1ebfn v\u1ecb chua v\u00e0 m\u00f9i kh\u00f3 ch\u1ecbu trong bia.<\/p>\n
  Qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n h\u1ec7 th\u1ed1ng x\u00e9t nghi\u1ec7m BREWPAL bao g\u1ed3m m\u1ed9t quy tr\u00ecnh g\u1ed3m ba giai \u0111o\u1ea1n m\u1edf r\u1ed9ng: thi\u1ebft k\u1ebf m\u1ed3i, t\u1ed1i \u01b0u h\u00f3a m\u1ed3i v\u00e0 t\u1ed1i \u01b0u h\u00f3a PCR.<\/p>\n
  C\u00e1c gen kh\u00e1ng hoa bia horA v\u00e0 horC \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 c\u00e1c marker di truy\u1ec1n t\u1ea1o ra Lactobacillus v\u00e0 Pediococcus spp, c\u00f3 kh\u1ea3 n\u0103ng l\u00e0m h\u1ecfng bia. S\u1ea3n ph\u1ea9m gen c\u1ee7a horA gi\u00fap kh\u00e1ng hoa bia v\u1edbi LAB b\u1eb1ng c\u00e1ch ho\u1ea1t \u0111\u1ed9ng nh\u01b0 m\u1ed9t ch\u1ea5t v\u1eadn chuy\u1ec3n \u0111a thu\u1ed1c, ph\u1ee5 thu\u1ed9c ATP gi\u00fap lo\u1ea1i b\u1ecf axit \u0111\u1eafng hoa bia ra kh\u1ecfi t\u1ebf b\u00e0o trong khi horC m\u00e3 h\u00f3a m\u1ed9t ch\u1ea5t v\u1eadn chuy\u1ec3n \u0111a thu\u1ed1c, ph\u1ee5 thu\u1ed9c v\u00e0o l\u1ef1c, c\u00f3 \u0111\u1ed9ng c\u01a1 proton. Trong m\u1ed9t nghi\u00ean c\u1ee9u to\u00e0n di\u1ec7n tr\u00ean 50 sinh v\u1eadt, ng\u01b0\u1eddi ta \u0111\u00e3 ch\u1ee9ng minh r\u1eb1ng 94% Lactobacillus BREWBRUX\u00ae Brettanomyces bruxellensis v\u00e0 lo\u00e0i Pediococcus th\u1eed nghi\u1ec7m c\u00f3 horA, trong khi 96% c\u00f3 horC. BREWPAL nh\u1eafm v\u00e0o c\u1ea3 c\u00e1c gen kh\u00e1ng hoa bia, \u0111\u1eb7c hi\u1ec7u c\u1ee7a Lactobacillus v\u00e0 Pediococcus \u2014\u0111\u1ea3m b\u1ea3o 100% c\u00e1c lo\u00e0i \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n v\u00e0 lo\u1ea1i b\u1ecf kh\u1ea3 n\u0103ng x\u1ea3y ra k\u1ebft qu\u1ea3 \u00e2m t\u00ednh gi\u1ea3.<\/p>\n
  T\u1ed5ng c\u1ed9ng c\u00f3 42 c\u1eb7p m\u1ed3i nguy\u00ean m\u1eabu (20 m\u1ed3i m\u1ee5c ti\u00eau horA v\u00e0 22 m\u1ed3i m\u1ee5c ti\u00eau horC) \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch trong m\u1ed9t h\u1ec7 th\u1ed1ng gh\u00e9p k\u00eanh \u0111\u1ec3 ch\u1ecdn ra c\u00e1c c\u1eb7p \u1ee9ng c\u1eed vi\u00ean m\u1ed3i h\u00e0ng \u0111\u1ea7u trong \u0111\u00f3 c\u00e1c m\u1ee5c ti\u00eau horA v\u00e0 horC \u0111\u01b0\u1ee3c khu\u1ebfch \u0111\u1ea1i \u0111\u1ed3ng th\u1eddi.<\/p>\n
  $\"\"$
  B\u1ea3ng 2:<\/strong> T\u00f3m t\u1eaft qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n BREWPAL\u00ae n\u1ed9i b\u1ed9<\/figcaption><\/figure>\n
  C\u00e1c nghi\u00ean c\u1ee9u to\u00e0n di\u1ec7n v\u00e0 \u0111\u1ed9c quy\u1ec1n \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a v\u00e0 x\u00e1c nh\u1eadn \u0111\u1ed9 nh\u1ea1y x\u00e9t nghi\u1ec7m c\u1ee7a h\u1ec7 th\u1ed1ng. T\u1ed5ng c\u1ed9ng c\u00f3 21 ch\u1ee7ng Lactobacillus v\u00e0 Pediococcus v\u00e0 31 vi sinh v\u1eadt c\u00f3 li\u00ean quan ch\u1eb7t ch\u1ebd \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb bia \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng l\u1ea7n l\u01b0\u1ee3t cho c\u00e1c nghi\u00ean c\u1ee9u bao g\u1ed3m v\u00e0 \u0111\u1ed9c quy\u1ec1n. M\u1ed9t b\u1ed9 \u0111\u1ea7y \u0111\u1ee7 g\u1ed3m 67 b\u1ea3ng \u0111\u1ed9 nh\u1ea1y \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n (LOD) c\u1ee7a x\u00e9t nghi\u1ec7m. C\u00e1c n\u1ec1n m\u1eabu \u0111a d\u1ea1ng \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m trong su\u1ed1t qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n, bao g\u1ed3m bia nh\u1ea1t v\u00e0 \u0111\u1eadm, bia \u0111\u1eb7c bi\u1ec7t v\u00e0 bia c\u00f3 h\u01b0\u01a1ng v\u1ecb, n\u1ed3ng \u0111\u1ed9 c\u1ed3n cao v\u00e0 bia l\u00ean men nhi\u1ec1u l\u1ea7n c\u0169ng nh\u01b0 c\u00e1c m\u1eabu thu \u0111\u01b0\u1ee3c \u1edf c\u00e1c giai \u0111o\u1ea1n kh\u00e1c nhau c\u1ee7a qu\u00e1 tr\u00ecnh l\u00ean men. Trong qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n v\u00e0 x\u00e1c nh\u1eadn n\u1ed9i b\u1ed9\/b\u00ean ngo\u00e0i, h\u01a1n 1300 cassette BREWPAL \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ea1y tr\u00ean nhi\u1ec1u m\u1eabu v\u00e0 c\u00e1c matrix kh\u00e1c nhau \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o \u0111\u00e1p \u1ee9ng c\u00e1c ti\u00eau ch\u00ed hi\u1ec7u su\u1ea5t. Ngo\u00e0i vi\u1ec7c x\u00e1c nh\u1eadn n\u1ed9i b\u1ed9, h\u1ec7 th\u1ed1ng BREWPAL c\u00f2n ph\u1ea3i ch\u1ecbu c\u00e1c nghi\u00ean c\u1ee9u x\u00e1c nh\u1eadn b\u00ean ngo\u00e0i \u0111\u1ec3 ki\u1ec3m tra \u0111\u1ed9 nh\u1ea1y, \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 hi\u1ec7u su\u1ea5t t\u1eeb c\u00e1c m\u1eabu c\u1ee7a kh\u00e1ch h\u00e0ng. D\u1eef li\u1ec7u \u0111\u01b0\u1ee3c thu th\u1eadp cho \u0111\u1ebfn nay \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y chi ti\u1ebft trong c\u00e1c ph\u1ea7n sau.<\/p>\n
  $\"\"$
  B\u1ea3ng 3:<\/strong> Th\u00f4ng s\u1ed1 hi\u1ec7u su\u1ea5t c\u1ee7a BREWPAL s\u1eed d\u1ee5ng VERIFLOW\u00ae<\/figcaption><\/figure>\n
  M\u1ed9t nghi\u00ean c\u1ee9u x\u00e1c nh\u1eadn n\u1ed9i b\u1ed9 \u0111\u00e3 \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh tr\u01b0\u1edbc khi ra m\u1eaft BREWPAL\u00ae . C\u00e1c m\u1eabu bia t\u1eeb ba nh\u00e0 m\u00e1y bia bao g\u1ed3m c\u00e1c lo\u1ea1i bia kh\u00e1c nhau \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a vi khu\u1ea9n Lactobacillus ho\u1eb7c Pediococcus g\u00e2y h\u01b0 h\u1ecfng b\u1eb1ng x\u00e9t nghi\u1ec7m BREWPAL. D\u1eef li\u1ec7u ch\u1ee9ng minh t\u00ednh b\u1ec1n v\u1eefng v\u00e0 kh\u1ea3 n\u0103ng t\u01b0\u01a1ng th\u00edch ma tr\u1eadn c\u1ee7a h\u1ec7 th\u1ed1ng BREWPAL, cho ph\u00e9p ph\u00e2n t\u00edch nhi\u1ec1u lo\u1ea1i s\u1ea3n ph\u1ea9m bia.<\/p>\n
  $\"\"$
  B\u1ea3ng 4: T\u00f3m t\u1eaft k\u1ebft qu\u1ea3 x\u00e1c th\u1ef1c n\u1ed9i b\u1ed9<\/figcaption><\/figure>\n
  <\/span>X\u00e1c th\u1ef1c b\u00ean ngo\u00e0i<\/strong><\/span><\/h3>\n<\/span>C\u00e1c th\u1eed nghi\u1ec7m kh\u00e1c trong Danh m\u1ee5c Bia<\/strong><\/span><\/h4>\n
  Quy tr\u00ecnh ph\u00e1t tri\u1ec3n x\u00e9t nghi\u1ec7m c\u1ed1t l\u00f5i cho t\u1eebng b\u1ed9 d\u1ee5ng c\u1ee5 t\u01b0\u01a1ng t\u1ef1 nh\u01b0 quy tr\u00ecnh c\u1ee7a BREWPAL. Qu\u00e1 tr\u00ecnh n\u00e0y bao g\u1ed3m ba giai \u0111o\u1ea1n ch\u00ednh. Giai \u0111o\u1ea1n \u0111\u1ea7u ti\u00ean bao g\u1ed3m thi\u1ebft k\u1ebf m\u1ed3i, sau \u0111\u00f3 l\u00e0 s\u00e0ng l\u1ecdc c\u00e1c c\u1eb7p m\u1ed3i \u0111\u00f3.<\/p>\n
  Sau \u0111\u00f3, c\u00e1c m\u1ed3i h\u00e0ng \u0111\u1ea7u th\u1ec3 hi\u1ec7n kh\u1ea3 n\u0103ng khu\u1ebfch \u0111\u1ea1i m\u1eabu m\u1ee5c ti\u00eau c\u00f3 \u0111\u1ed9 ch\u00ednh x\u00e1c cao trong giai \u0111o\u1ea1n th\u1ee9 hai c\u1ee7a quy tr\u00ecnh \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch trong c\u00e1c n\u1ec1n m\u1eabu bia kh\u00e1c nhau v\u1edbi m\u1ee9c \u0111\u1ed9 hoa bia v\u00e0 r\u01b0\u1ee3u kh\u00e1c nhau \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9 nh\u1ea1y khi c\u00f3 m\u1eb7t c\u00e1c h\u1ee3p ch\u1ea5t \u1ee9c ch\u1ebf. Cu\u1ed1i c\u00f9ng, nhi\u1ec1u b\u1ea3ng LOD (gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n) \u0111\u00e3 \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh trong bia \u0111\u1ec3 x\u00e1c nh\u1eadn \u0111\u1ed9 nh\u1ea1y c\u1ee7a x\u00e9t nghi\u1ec7m, sau \u0111\u00f3 l\u00e0 c\u00e1c b\u1ea3ng bao g\u1ed3m v\u00e0 \u0111\u1ed9c quy\u1ec1n \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 t\u00ednh \u0111\u1eb7c hi\u1ec7u c\u1ee7a c\u1ea5u h\u00ecnh b\u1ed9 c\u00f4ng c\u1ee5 cu\u1ed1i c\u00f9ng. Trong qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n, h\u00e0ng tr\u0103m cassette \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ea1y tr\u00ean nhi\u1ec1u m\u1eabu v\u00e0 matrix kh\u00e1c nhau \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o \u0111\u00e1p \u1ee9ng c\u00e1c ti\u00eau ch\u00ed v\u1ec1 hi\u1ec7u su\u1ea5t.<\/p>\n
  $\"\"$
  B\u1ea3ng 5: T\u00f3m t\u1eaft qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n x\u00e9t nghi\u1ec7m cho d\u00f2ng VERIFLOW\u00ae c\u00f2n l\u1ea1i<\/figcaption><\/figure>\n
  $\"\"$ <\/p>\n
  Danh m\u1ee5c bia VERIFLOW\u00ae<\/a> \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3 ph\u00e1t hi\u1ec7n tr\u00ecnh t\u1ef1 DNA ribosome \u0111\u01b0\u1ee3c b\u1ea3o t\u1ed3n t\u1eeb nhi\u1ec1u lo\u1ea1i bia h\u1ecfng nh\u01b0 \u0111\u01b0\u1ee3c li\u1ec7t k\u00ea trong B\u1ea3ng 6. T\u1ea5t c\u1ea3 c\u00e1c x\u00e9t nghi\u1ec7m \u0111\u1ec1u \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3 c\u00f3 ng\u01b0\u1ee1ng ph\u00e1t hi\u1ec7n l\u00e0 10 t\u1ebf b\u00e0o\/mL v\u1edbi th\u1eddi gian quay v\u00f2ng ba gi\u1edd ( ngo\u1ea1i tr\u1eeb BREWSTAT y\u00eau c\u1ea7u b\u1ed1n gi\u1edd). To\u00e0n b\u1ed9 danh m\u1ee5c c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u1edbi nhi\u1ec1u lo\u1ea1i bia, PCR khu\u1ea9n l\u1ea1c v\u00e0 m\u1eabu m\u00f4i tr\u01b0\u1eddng. Ngo\u00e0i ra, m\u1ed9t s\u1ed1 th\u1eed nghi\u1ec7m \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c nh\u1eadn v\u00e0 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t hi\u1ec7n c\u00e1c lo\u1ea1i n\u1ea5m men g\u00e2y h\u01b0 h\u1ecfng. Ngo\u1ea1i tr\u1eeb BREWDEK\u00ae v\u00e0 BREWSTAT, t\u1ea5t c\u1ea3 c\u00e1c th\u1eed nghi\u1ec7m \u0111\u1ec1u \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3 t\u01b0\u01a1ng th\u00edch v\u1edbi \u0110\u1ea7u \u0111\u1ecdc quang \u0111\u1ec3 c\u00f3 k\u1ebft qu\u1ea3 \u0111\u1ecbnh l\u01b0\u1ee3ng.<\/p>\n
  <\/span>B\u1ea2N T\u00d3M T\u1eaeT<\/strong><\/span><\/h2>\n
  Ng\u00e0nh c\u00f4ng nghi\u1ec7p s\u1ea3n xu\u1ea5t bia \u0111\u00e3 ph\u00e1t tri\u1ec3n theo c\u1ea5p s\u1ed1 nh\u00e2n trong th\u1eadp k\u1ef7 qua v\u1edbi nh\u1eefng ng\u01b0\u1eddi m\u1edbi tham gia tr\u00e0n ng\u1eadp th\u1ecb tr\u01b0\u1eddng. Do s\u1ef1 \u0111a d\u1ea1ng c\u1ee7a c\u00e1c phong c\u00e1ch bia v\u00e0 \u0111\u1ed9 ph\u1ee9c t\u1ea1p trong qu\u00e1 tr\u00ecnh s\u1ea3n xu\u1ea5t bia, qu\u1ea3n l\u00fd ch\u1ea5t l\u01b0\u1ee3ng \u0111\u00e3 tr\u1edf th\u00e0nh \u0111i\u1ec3m kh\u00e1c bi\u1ec7t quan tr\u1ecdng h\u01a1n bao gi\u1edd h\u1ebft. Nhu c\u1ea7u v\u1ec1 th\u00f4ng tin ch\u00ednh x\u00e1c, h\u1eefu \u00edch trong qu\u00e1 tr\u00ecnh s\u1ea3n xu\u1ea5t v\u00e0 sau khi \u0111\u00f3ng g\u00f3i l\u00e0 \u0111i\u1ec1u c\u1ea7n thi\u1ebft \u0111\u1ec3 duy tr\u00ec ch\u1ea5t l\u01b0\u1ee3ng v\u00e0 b\u1ea3o v\u1ec7 h\u00ecnh \u1ea3nh th\u01b0\u01a1ng hi\u1ec7u ng\u00e0y nay.<\/p>\n
  C\u00e1c ph\u01b0\u01a1ng ph\u00e1p ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng truy\u1ec1n th\u1ed1ng, n\u1ebfu \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u0111\u00fang c\u00e1ch, c\u00f3 th\u1ec3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c v\u1ea5n \u0111\u1ec1, nh\u01b0ng ch\u00fang kh\u00f4ng th\u1ec3 th\u1ef1c hi\u1ec7n nhanh ch\u00f3ng v\u00e0 hi\u1ec7u qu\u1ea3. Th\u00f4ng tin b\u1ecb tr\u00ec ho\u00e3n d\u1eabn \u0111\u1ebfn c\u00e1c quy\u1ebft \u0111\u1ecbnh v\u1ec1 ch\u1ea5t l\u01b0\u1ee3ng mang t\u00ednh ph\u1ea3n \u1ee9ng \u0111\u01b0\u1ee3c \u0111\u01b0a ra sau khi thi\u1ec7t h\u1ea1i \u0111\u00e3 x\u1ea3y ra. Ngo\u00e0i ra, nh\u1eefng c\u00f4ng ngh\u1ec7 n\u00e0y th\u01b0\u1eddng y\u00eau c\u1ea7u quy tr\u00ecnh l\u00e0m vi\u1ec7c r\u01b0\u1eddm r\u00e0, t\u1ed1n k\u00e9m v\u00e0 ph\u1ee9c t\u1ea1p khi s\u1eed d\u1ee5ng. Ngo\u00e0i ra, k\u1ebft qu\u1ea3 th\u1eed nghi\u1ec7m c\u00f3 th\u1ec3 kh\u00f4ng r\u00f5 r\u00e0ng v\u1ec1 nguy c\u01a1 h\u01b0 h\u1ecfng c\u1ee7a m\u1ed9t vi sinh v\u1eadt c\u1ee5 th\u1ec3. Vi\u1ec7c gi\u1ea3i quy\u1ebft c\u00e1c sinh v\u1eadt g\u00e2y h\u01b0 h\u1ecfng trong th\u1eddi gian th\u1ef1c l\u00e0 r\u1ea5t quan tr\u1ecdng \u0111\u1ec3 tr\u00e1nh \u00f4 nhi\u1ec5m c\u01a1 s\u1edf, l\u01b0u gi\u1eef s\u1ea3n ph\u1ea9m ho\u1eb7c v\u1eadn chuy\u1ec3n bia c\u00f3 nguy c\u01a1.<\/p>\n
  \u0110\u1ec3 gi\u1ea3i quy\u1ebft nh\u1eefng th\u00e1ch th\u1ee9c n\u00e0y, INVISIBLE SENTINEL<\/a> \u0111\u00e3 ph\u00e1t tri\u1ec3n BREWPAL\u00ae v\u00e0 c\u00e1c x\u00e9t nghi\u1ec7m kh\u00e1c \u0111\u1ec3 cung c\u1ea5p c\u00e1c x\u00e9t nghi\u1ec7m nhanh ch\u00f3ng, gi\u00e1 c\u1ea3 ph\u1ea3i ch\u0103ng v\u00e0 th\u00e2n thi\u1ec7n v\u1edbi ng\u01b0\u1eddi d\u00f9ng \u2014 m\u00e0 kh\u00f4ng l\u00e0m gi\u1ea3m \u0111\u1ed9 nh\u1ea1y, \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u ho\u1eb7c \u0111\u1ed9 ch\u00ednh x\u00e1c. Vi\u1ec7c ph\u00e2n t\u00edch c\u00e1c n\u1ec1n m\u1eabu th\u1eed th\u00e1ch m\u00e0 kh\u00f4ng c\u1ea7n t\u00e1ch chi\u1ebft v\u00e0\/ho\u1eb7c tinh ch\u1ebf DNA, ch\u1ec9 c\u00f3 th\u1ec3 th\u1ef1c hi\u1ec7n \u0111\u01b0\u1ee3c v\u1edbi VERIFLOW\u00ae<\/a>, gi\u00fap gi\u1ea3m th\u1eddi gian x\u1eed l\u00fd m\u1eabu v\u00e0 chi ph\u00ed v\u1eadt li\u1ec7u. BREWPAL v\u00e0 c\u00e1c s\u1ea3n ph\u1ea9m bia ho\u00e0n ch\u1ec9nh d\u1ef1a tr\u00ean VERIFLOW<\/a> \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00e1c nh\u00e0 m\u00e1y bia h\u00e0ng \u0111\u1ea7u tr\u00ean th\u1ebf gi\u1edbi \u00e1p d\u1ee5ng r\u1ed9ng r\u00e3i v\u00e0 c\u00f4ng ngh\u1ec7 n\u00e0y \u0111\u00e3 nhanh ch\u00f3ng tr\u1edf th\u00e0nh ph\u01b0\u01a1ng ph\u00e1p h\u00e0ng \u0111\u1ea7u trong ng\u00e0nh \u0111\u1ec3 th\u1eed nghi\u1ec7m vi sinh v\u1eadt. BREWPAL v\u00e0 danh m\u1ee5c s\u1ea3n ph\u1ea9m ho\u00e0n ch\u1ec9nh d\u1ef1a tr\u00ean VERIFLOW<\/a> nh\u1eafm \u0111\u1ebfn nhi\u1ec1u lo\u1ea1i sinh v\u1eadt kh\u00e1c nhau v\u00e0 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong su\u1ed1t qu\u00e1 tr\u00ecnh s\u1ea3n xu\u1ea5t bia, trong th\u00e0nh ph\u1ea9m v\u00e0 \u0111\u1ec3 th\u1eed nghi\u1ec7m m\u00f4i tr\u01b0\u1eddng.<\/p>\n
  $\"\"$ <\/p>\n
  Ngu\u1ed3n: https:\/\/invisiblesentinel.com\/site\/assets\/files\/1413\/beer-white-paper.pdf<\/a><\/em><\/p>\n
  Minh Khang l\u00e0 nh\u00e0 ph\u00e2n ph\u1ed1i v\u00e0 nh\u1eadp kh\u1ea9u tr\u1ef1c ti\u1ebfp thi\u1ebft b\u1ecb ki\u1ec3m so\u00e1t vi sinh trong bia VERIFLOW\u2122<\/a> h\u00e3ng Invisible Sentinel<\/a> (BioM\u00e9rieux<\/a>).<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"
  INVISIBLE SENTINEL \u0111\u00e3 ph\u00e1t tri\u1ec3n m\u1ed9t c\u00f4ng ngh\u1ec7 ph\u00e2n t\u1eed m\u1edbi \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 VERIFLOW, ki\u1ec3m so\u00e1t vi sinh v\u1eadt cho ng\u00e0nh bia b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p PCR, gi\u00fap c\u1ea3i thi\u1ec7n tri\u1ec7t \u0111\u1ec3 c\u00e1c v\u1ea5n \u0111\u1ec1 h\u1ea1n ch\u1ebf nh\u01b0 chi ph\u00ed cao, \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c th\u1ea5p. VERIFLOW c\u0169ng \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf v\u1edbi m\u1ed9t…<\/p>\n","protected":false},"author":3,"featured_media":10065,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"rank_math_lock_modified_date":false,"footnotes":""},"categories":[40],"tags":[],"class_list":["post-10064","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"acf":[],"_links":{"self":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10064"}],"collection":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/comments?post=10064"}],"version-history":[{"count":11,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10064\/revisions"}],"predecessor-version":[{"id":10089,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10064\/revisions\/10089"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media\/10065"}],"wp:attachment":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media?parent=10064"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/categories?post=10064"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/tags?post=10064"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}