\nTóm t\u1eaft n\u1ed9i dung<\/label>\n

Ly t\u00e2m h\u1ed7n h\u1ee3p protein th\u00f4 – B\u01b0\u1edbc b\u1eaft bu\u1ed9c trong nghi\u00ean c\u1ee9u tinh ch\u1ebf v\u00e0 t\u1ed5ng h\u1ee3p protein<\/a><\/li>
Si\u00eau ly t\u00e2m – B\u01b0\u1edbc m\u1ea1nh m\u1ebd \u0111\u1ec3 t\u00e1ch protein kh\u1ecfi c\u00e1c kh\u1ed1i d\u1ef1a tr\u00ean k\u00edch th\u01b0\u1edbc<\/a><\/li>
Si\u00eau ly t\u00e2m ph\u00e2n t\u00edch – M\u1ed9t c\u00f4ng c\u1ee5 m\u1ea1nh m\u1ebd \u0111\u1ec3 ph\u00e2n t\u00edch protein t\u1eeb c\u00e1c t\u1eadp h\u1ee3p<\/a><\/li><\/ul><\/nav><\/div>\n
Ly t\u00e2m<\/a> l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 m\u1ea1nh m\u1ebd cho quy tr\u00ecnh c\u00f4ng vi\u1ec7c \u0111\u1ec3 c\u00f4 l\u1eadp v\u00e0 x\u00e1c \u0111\u1ecbnh \u0111\u1eb7c t\u00ednh c\u1ee7a c\u00e1c s\u1ea3n ph\u1ea9m protein. S\u1ef1 t\u1eadp h\u1ee3p protein l\u00e0 m\u1ed9t hi\u1ec7n t\u01b0\u1ee3ng sinh h\u1ecdc g\u00e2y ra b\u1edfi s\u1ef1 t\u01b0\u01a1ng t\u00e1c b\u1ea5t th\u01b0\u1eddng c\u1ee7a c\u00e1c protein b\u1ecb m\u1ea5t \u1ed5n \u0111\u1ecbnh. \u0110\u1eb7c t\u00ednh ho\u1ea1t \u0111\u1ed9ng c\u1ee7a protein trong dung d\u1ecbch l\u00e0 r\u1ea5t quan tr\u1ecdng, v\u00ec c\u00e1c protein kh\u00f4ng \u1ed5n \u0111\u1ecbnh d\u1ec5 b\u1ecb thay \u0111\u1ed5i v\u1ec1 m\u1eb7t h\u00f3a h\u1ecdc v\u00e0 v\u1eadt l\u00fd d\u1eabn \u0111\u1ebfn m\u1ea5t ch\u1ee9c n\u0103ng. Quan tr\u1ecdng nh\u1ea5t, trong qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n thu\u1ed1c d\u1ef1a tr\u00ean protein, ngay c\u1ea3 l\u01b0\u1ee3ng t\u1eadp h\u1ee3p t\u1ed1i thi\u1ec3u c\u0169ng c\u00f3 th\u1ec3 g\u00e2y ra t\u00e1c \u0111\u1ed9ng c\u00f3 h\u1ea1i do thay \u0111\u1ed5i ti\u1ec1m n\u0103ng v\u1ec1 hi\u1ec7u l\u1ef1c c\u1ee7a d\u01b0\u1ee3c ph\u1ea9m sinh h\u1ecdc v\u00e0 ph\u1ea3n \u1ee9ng mi\u1ec5n d\u1ecbch b\u1ea5t l\u1ee3i \u1edf b\u1ec7nh nh\u00e2n. Ngo\u00e0i ra, s\u1ed1 l\u01b0\u1ee3ng thu\u1ed1c sinh h\u1ecdc t\u01b0\u01a1ng t\u1ef1 ng\u00e0y c\u00e0ng t\u0103ng \u0111\u00f2i h\u1ecfi c\u00e1c quy tr\u00ecnh qu\u1ea3n l\u00fd nghi\u00eam ng\u1eb7t nh\u1eb1m gi\u1ea3i quy\u1ebft h\u1ee3p l\u00fd vi\u1ec7c ph\u00e2n t\u00edch ch\u1ea5t l\u01b0\u1ee3ng s\u1ea3n ph\u1ea9m c\u1ee7a c\u00e1c c\u00f4ng ty d\u01b0\u1ee3c ph\u1ea9m sinh h\u1ecdc.<\/p>\n
Nhi\u1ec1u y\u1ebfu t\u1ed1 c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn ho\u1ea1t \u0111\u1ed9ng v\u00e0 s\u1ef1 k\u1ebft t\u1ee5 c\u1ee7a protein, bao g\u1ed3m nhi\u1ec7t \u0111\u1ed9, qu\u00e1 tr\u00ecnh oxy h\u00f3a, n\u1ed3ng \u0111\u1ed9, pH, c\u01b0\u1eddng \u0111\u1ed9 ion, ch\u1ea5t ph\u1ee5 gia, ch\u1ea5t t\u1ea9y r\u1eeda, ch\u1ea5t b\u1ea3o qu\u1ea3n l\u1ea1nh, chu tr\u00ecnh \u0111\u00f4ng l\u1ea1nh, r\u00e3 \u0111\u00f4ng, \u0111i\u1ec1u ki\u1ec7n b\u1ea3o qu\u1ea3n v\u00e0 x\u1eed l\u00fd.<\/p>\n
Danh m\u1ee5c m\u00e1y ly t\u00e2m<\/a> Beckman Coulter<\/a> mang \u0111\u1ebfn kh\u1ea3 n\u0103ng ph\u00e2n t\u00e1ch ch\u1ea5t l\u01b0\u1ee3ng v\u00e0 ph\u00e2n t\u00edch protein ti\u00ean ti\u1ebfn, mang \u0111\u1ebfn cho ng\u01b0\u1eddi d\u00f9ng c\u00e1c gi\u1ea3i ph\u00e1p to\u00e0n di\u1ec7n cho c\u1ea3 quy tr\u00ecnh. Gi\u1ea3i ph\u00e1p quy tr\u00ecnh l\u00e0m vi\u1ec7c d\u1ef1a tr\u00ean ly t\u00e2m<\/a> c\u00f3 th\u1ec3 c\u00f3 hi\u1ec7u qu\u1ea3 cao v\u00ec n\u00f3 gi\u1ea3i quy\u1ebft m\u1ed9t c\u00e1ch h\u1ec7 th\u1ed1ng nhu c\u1ea7u ph\u00e2n t\u00e1ch b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng \u0111\u1ed9 d\u1ed1c l\u1ef1c g v\u00e0 m\u1eadt \u0111\u1ed9 t\u0103ng d\u1ea7n \u0111\u1ec3 t\u00e1ch c\u00e1c h\u1ea1t d\u1ef1a tr\u00ean kh\u1ed1i l\u01b0\u1ee3ng v\u00e0 m\u1eadt \u0111\u1ed9.<\/p>\n
$\"ly$ <\/p>\n
M\u1ed9t chu\u1ed7i polypeptide ch\u01b0a \u0111\u01b0\u1ee3c g\u1eadp l\u1ea1i s\u1ebd t\u1ea1o th\u00e0nh c\u1ea5u tr\u00fac protein ba chi\u1ec1u \u0111\u01b0\u1ee3c g\u1eadp l\u1ea1i \u0111\u00fang c\u00e1ch. Ng\u01b0\u1ee3c l\u1ea1i, n\u1ebfu c\u00e1c t\u01b0\u01a1ng t\u00e1c ch\u00ednh kh\u00f4ng \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh, protein kh\u00f4ng \u1ed5n \u0111\u1ecbnh c\u00f3 th\u1ec3 t\u1ed5ng h\u1ee3p v\u00e0 t\u1ea1o th\u00e0nh c\u00e1c kh\u1ed1i protein \u0111\u1ed9c h\u1ea1i.<\/p>\n
<\/span>Ly t\u00e2m<\/a> h\u1ed7n h\u1ee3p protein th\u00f4 – B\u01b0\u1edbc b\u1eaft bu\u1ed9c trong nghi\u00ean c\u1ee9u tinh ch\u1ebf v\u00e0 t\u1ed5ng h\u1ee3p protein<\/span><\/h2>\n
Kh\u1edfi \u0111\u1ea7u c\u1ee7a nghi\u00ean c\u1ee9u v\u1ec1 s\u1ef1 t\u1ed5ng h\u1ee3p protein l\u00e0 qu\u00e1 tr\u00ecnh tinh ch\u1ebf protein. Th\u00f4ng th\u01b0\u1eddng, protein \u0111\u01b0\u1ee3c bi\u1ec3u hi\u1ec7n qu\u00e1 m\u1ee9c trong c\u01a1 th\u1ec3 sinh v\u1eadt, nh\u01b0 vi khu\u1ea9n, n\u1ea5m men ho\u1eb7c t\u1ebf b\u00e0o \u0111\u1ed9ng v\u1eadt c\u00f3 v\u00fa trong m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y. C\u00e1c \u0111\u1eb7c \u0111i\u1ec3m ri\u00eang c\u1ee7a m\u1ed7i protein, ch\u1eb3ng h\u1ea1n nh\u01b0 th\u00e0nh ph\u1ea7n axit amin, k\u00edch th\u01b0\u1edbc, h\u00ecnh d\u1ea1ng, \u0111i\u1ec3m \u0111\u1eb3ng \u0111i\u1ec7n v\u00e0 \u0111\u1ed9 h\u00f2a tan \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t tri\u1ec3n c\u00e1c chi\u1ebfn l\u01b0\u1ee3c ri\u00eang bi\u1ec7t \u0111\u1ec3 ph\u00e2n l\u1eadp protein quan t\u00e2m.\u00a0M\u1ee5c ti\u00eau l\u00e0 c\u00f4 l\u1eadp l\u01b0\u1ee3ng protein ch\u1ee9c n\u0103ng quan t\u00e2m l\u1edbn nh\u1ea5t v\u1edbi l\u01b0\u1ee3ng ch\u1ea5t g\u00e2y \u00f4 nhi\u1ec5m kh\u00e1c \u00edt nh\u1ea5t.\u00a0 Ly t\u00e2m<\/a> l\u00e0 b\u01b0\u1edbc quan tr\u1ecdng v\u00e0 th\u01b0\u1eddng l\u00e0 b\u01b0\u1edbc \u0111\u1ea7u ti\u00ean trong b\u1ea5t k\u1ef3 quy tr\u00ecnh tinh ch\u1ebf protein n\u00e0o.\u00a0D\u00f2ng m\u00e1y ly t\u00e2m t\u1ed1c \u0111\u1ed9 cao<\/a> Avanti JXN<\/a> c\u00f3 s\u1eb5n m\u1ed9t lo\u1ea1t r\u00f4to \u0111\u1ec3 h\u1ed7 tr\u1ee3 b\u1ea5t k\u1ef3 giai \u0111o\u1ea1n tinh ch\u1ebf protein n\u00e0o. Nhi\u1ec1u s\u1ef1 l\u1ef1a ch\u1ecdn rotor – v\u1edbi s\u1ef1 k\u1ebft h\u1ee3p kh\u00e1c nhau v\u1ec1 \u00e2m l\u01b0\u1ee3ng v\u00e0 l\u1ef1c g – l\u00e0m cho JXN<\/a> tr\u1edf th\u00e0nh m\u1ed9t thi\u1ebft b\u1ecb linh ho\u1ea1t c\u00f3 kh\u1ea3 n\u0103ng \u0111\u00e1p \u1ee9ng nhu c\u1ea7u c\u1ee7a m\u1ed9t lo\u1ea1t c\u00e1c d\u1ef1 \u00e1n proteomic v\u00e0 c\u00f3 th\u1ec3 tu\u00e2n theo c\u00e1c b\u1ed9 \u0111\u1ec7m kh\u00e1c nhau \u0111\u1ec3 th\u00fac \u0111\u1ea9y s\u1ef1 \u1ed5n \u0111\u1ecbnh c\u1ee7a protein. Tinh ch\u1ebf protein ly t\u00e2m v\u1edbi d\u00f2ng Avanti JXN<\/a> hi\u1ec7u su\u1ea5t cao r\u1ea5t m\u1ea1nh m\u1ebd v\u00e0 cung c\u1ea5p cho ng\u01b0\u1eddi d\u00f9ng k\u1ef9 thu\u1eadt c\u00f3 kh\u1ea3 n\u0103ng t\u00e1i s\u1ea3n xu\u1ea5t cao.<\/p>\n
<\/span>Si\u00eau ly t\u00e2m<\/a> – B\u01b0\u1edbc m\u1ea1nh m\u1ebd \u0111\u1ec3 t\u00e1ch protein kh\u1ecfi c\u00e1c kh\u1ed1i d\u1ef1a tr\u00ean k\u00edch th\u01b0\u1edbc<\/span><\/h2>\n
Ng\u01b0\u1eddi ta \u0111\u00e3 ch\u1ee9ng minh r\u00f5 r\u00e0ng r\u1eb1ng ly t\u00e2m<\/a> l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p m\u1ea1nh m\u1ebd v\u00e0 c\u00f3 th\u1ec3 \u00e1p d\u1ee5ng r\u1ed9ng r\u00e3i \u0111\u1ec3 t\u00e1ch h\u1ed7n h\u1ee3p th\u00f4 c\u1ee7a c\u00e1c th\u00e0nh ph\u1ea7n t\u1ebf b\u00e0o. H\u01a1n n\u1eefa, si\u00eau ly t\u00e2m<\/a> k\u1ebft h\u1ee3p v\u1edbi gradient m\u1eadt \u0111\u1ed9 l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ch\u00ednh \u0111\u1ec3 t\u00e1ch v\u00e0 ph\u00e2n t\u00edch c\u00e1c protein quan t\u00e2m d\u1ef1a tr\u00ean k\u00edch th\u01b0\u1edbc v\u00e0 kh\u1ed1i l\u01b0\u1ee3ng. Trong qu\u00e1 tr\u00ecnh quay ly t\u00e2m<\/a> d\u01b0\u1edbi m\u1ed9t l\u1ef1c h\u1ea5p d\u1eabn c\u1ee5 th\u1ec3, c\u00e1c ph\u00e2n t\u1eed di chuy\u1ec3n t\u1eeb tr\u00ean xu\u1ed1ng d\u01b0\u1edbi c\u00f9ng c\u1ee7a dung d\u1ecbch trong \u1ed1ng.\u00a0Chuy\u1ec3n \u0111\u1ed9ng n\u00e0y \u0111\u01b0\u1ee3c b\u00f9 \u0111\u1eafp b\u1edfi m\u1eadt \u0111\u1ed9 v\u00e0 \u0111\u1ed9 nh\u1edbt c\u1ee7a ch\u1ea5t l\u1ecfng ho\u1eb7c l\u1ef1c n\u1ed5i.\u00a0\u1ede tr\u1ea1ng th\u00e1i c\u00e2n b\u1eb1ng, t\u00e1c \u0111\u1ed9ng r\u00f2ng c\u1ee7a l\u1ef1c h\u1ea5p d\u1eabn v\u00e0 l\u1ef1c n\u1ed5i s\u1ebd d\u1eabn \u0111\u1ebfn s\u1ef1 t\u00edch t\u1ee5 c\u00e1c protein c\u1ee5 th\u1ec3 t\u1ea1i m\u1ed9t \u0111i\u1ec3m c\u1ee5 th\u1ec3 trong \u1ed1ng, t\u1eeb \u0111\u00f3 t\u00e1ch protein ra kh\u1ecfi c\u00e1c t\u1eadp h\u1ee3p c\u00f3 h\u1ec7 s\u1ed1 l\u1eafng kh\u00e1c nhau. C\u00e1c protein t\u1ed5ng h\u1ee3p \u0111\u1eb7c h\u01a1n di chuy\u1ec3n ra ngo\u00e0i nhanh h\u01a1n c\u00e1c ph\u00e2n t\u1eed protein ri\u00eang l\u1ebb nh\u1eb9 h\u01a1n.<\/p>\n
\u0110\u1ed9 d\u1ed1c m\u1eadt \u0111\u1ed9 v\u00f9ng t\u1ef7 l\u1ec7 li\u00ean t\u1ee5c \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh b\u1eb1ng c\u00e1ch x\u1ebfp l\u1edbp \u0111\u1ed9 d\u1ed1c b\u01b0\u1edbc \u0111\u1ea7u ti\u00ean. Trong k\u1ef9 thu\u1eadt ph\u1ed5 bi\u1ebfn nh\u1ea5t, tr\u01b0\u1edbc ti\u00ean m\u1ed9t l\u01b0\u1ee3ng dung d\u1ecbch \u00edt \u0111\u1eadm \u0111\u1eb7c h\u01a1n \u0111\u01b0\u1ee3c b\u01a1m v\u00e0o \u1ed1ng ly t\u00e2m v\u00e0 c\u00e1c dung d\u1ecbch \u0111\u1eadm \u0111\u1eb7c h\u01a1n l\u1ea7n l\u01b0\u1ee3t \u0111\u01b0\u1ee3c \u0111\u01b0a v\u00e0o \u0111\u00e1y \u1ed1ng b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng m\u1ed9t \u1ed1ng ti\u00eam d\u00e0i \u0111\u1ec3 kh\u00f4ng l\u00e0m \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn l\u1edbp tr\u01b0\u1edbc \u0111\u00f3, \u0111\u1ec3 l\u1ea1i m\u1ed9t ranh gi\u1edbi r\u00f5 r\u00e0ng gi\u1eefa c\u00e1c l\u1edbp m\u1eadt \u0111\u1ed9 kh\u00e1c nhau. M\u1ed9t c\u00e1ch ti\u1ebfp c\u1eadn kh\u00e1c l\u00e0 x\u1ebfp l\u1edbp c\u00e1c gi\u1ea3i ph\u00e1p \u0111\u1eadm \u0111\u1eb7c gi\u1ea3m d\u1ea7n m\u1ed9t c\u00e1ch nh\u1eb9 nh\u00e0ng l\u00ean tr\u00ean c\u00e1c gi\u1ea3i ph\u00e1p \u0111\u1eadm \u0111\u1eb7c h\u01a1n. M\u1ed9t gradient li\u00ean t\u1ee5c c\u0169ng c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c t\u1ea1o ra t\u1eeb m\u1ed9t gradient kh\u00f4ng li\u00ean t\u1ee5c b\u1eb1ng c\u00e1ch \u1ee7 ho\u1eb7c quay dung d\u1ecbch trong m\u1ed9t kho\u1ea3ng th\u1eddi gian \u0111\u1ecbnh tr\u01b0\u1edbc ho\u1eb7c b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng m\u00e1y t\u1ea1o gradient. Ph\u01b0\u01a1ng ph\u00e1p th\u1ee9 ba, gradient m\u1eadt \u0111\u1ed9 n\u1ed5i, bao g\u1ed3m qu\u00e1 tr\u00ecnh ly t\u00e2m<\/a> t\u1ed1c \u0111\u1ed9 cao k\u00e9o d\u00e0i c\u1ee7a m\u1ed9t ph\u00e2n t\u1eed \u1edf m\u1eadt \u0111\u1ed9 dung d\u1ecbch t\u01b0\u01a1ng t\u1ef1, d\u1eabn \u0111\u1ebfn s\u1ef1 ph\u00e2n t\u00e1ch ph\u00e2n t\u1eed nghi\u00eam ng\u1eb7t.<\/p>\n
Trong t\u1ea5t c\u1ea3 c\u00e1c k\u1ef9 thu\u1eadt n\u00e0y, dung d\u1ecbch khu\u1ebfch t\u00e1n sao cho m\u1eadt \u0111\u1ed9 t\u0103ng d\u1ea7n t\u1eeb \u0111\u1ec9nh xu\u1ed1ng \u0111\u00e1y \u1ed1ng.<\/p>\n
Sau khi h\u00ecnh th\u00e0nh gradient, m\u1ed9t th\u1ec3 t\u00edch nh\u1ecf dung d\u1ecbch ch\u1ee9a h\u1ed7n h\u1ee3p protein \u0111\u01b0\u1ee3c \u0111\u1eb7t l\u00ean tr\u00ean gradient m\u1eadt \u0111\u1ed9.\u00a0H\u1ea7u nh\u01b0 lu\u00f4n lu\u00f4n, khi r\u00f4to quay, c\u00e1c protein di chuy\u1ec3n theo gradient v\u00e0 ph\u00e2n t\u00e1ch theo h\u1ec7 s\u1ed1 l\u1eafng c\u1ee7a ch\u00fang, v\u1edbi c\u00e1c t\u1eadp h\u1ee3p protein l\u1eafng nhanh h\u01a1n m\u1ed9t s\u1ea3n ph\u1ea9m protein ri\u00eang l\u1ebb \u0111\u01b0\u1ee3c quan t\u00e2m.\u00a0Th\u1eddi gian v\u00e0 t\u1ed1c \u0111\u1ed9 ly t\u00e2m \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh theo kinh nghi\u1ec7m.<\/p>\n
Sau khi ly t\u00e2m<\/a>, c\u00e1c protein t\u00e1ch bi\u1ec7t th\u01b0\u1eddng \u0111\u01b0\u1ee3c thu ho\u1ea1ch b\u1eb1ng c\u00e1ch l\u00e0m th\u1ee7ng m\u1ed9t l\u1ed7 \u1edf \u0111\u00e1y \u1ed1ng v\u00e0 thu t\u1eebng gi\u1ecdt c\u00e1c ph\u1ea7n ph\u00e2n \u0111o\u1ea1n, ho\u1eb7c b\u1eb1ng c\u00e1ch d\u00f9ng pipet l\u1ea5y c\u00e1c ph\u1ea7n ph\u00e2n \u0111o\u1ea1n t\u1eeb m\u1eb7t khum theo c\u00e1ch th\u1ee7 c\u00f4ng. C\u00e1c x\u00e9t nghi\u1ec7m sinh h\u00f3a ho\u1eb7c ch\u1ee9c n\u0103ng c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 h\u00e0m l\u01b0\u1ee3ng protein c\u1ee7a t\u1eebng ph\u1ea7n.<\/p>\n
Ngo\u00e0i vi\u1ec7c t\u00e1ch t\u1eadp h\u1ee3p protein, vi\u1ec7c tinh ch\u1ebf ph\u1ee9c h\u1ee3p protein-ph\u1ed1i t\u1eed th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong nghi\u00ean c\u1ee9u c\u00e1c li\u1ec7u ph\u00e1p \u0111i\u1ec1u tr\u1ecb d\u1ef1a tr\u00ean protein.\u00a0D\u00f2ng m\u00e1y si\u00eau ly t\u00e2m<\/a> Optima XPN<\/a> bao g\u1ed3m c\u00e1c m\u00e1y ly t\u00e2m<\/a> chu\u1ea9n b\u1ecb t\u01b0\u01a1ng th\u00edch v\u1edbi m\u1ed9t lo\u1ea1t r\u00f4to v\u00e0 v\u1eadt t\u01b0 ti\u00eau hao ph\u00f9 h\u1ee3p v\u1edbi nhi\u1ec1u giao th\u1ee9c kh\u00e1c nhau trong quy tr\u00ecnh tinh ch\u1ebf protein.<\/p>\n
M\u1ed9t k\u1ef9 thu\u1eadt th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u00e1ch protein l\u00e0 s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC). Khi so s\u00e1nh v\u1edbi qu\u00e1 tr\u00ecnh ly t\u00e2m<\/a>, SEC y\u00eau c\u1ea7u h\u1ec7 s\u1ed1 pha lo\u00e3ng l\u1edbn, t\u00ednh kh\u00f4ng t\u01b0\u01a1ng th\u00edch c\u1ee7a protein v\u00e0 c\u00e1c t\u01b0\u01a1ng t\u00e1c kh\u00f4ng mong mu\u1ed1n gi\u1eefa protein v\u00e0 nh\u1ef1a (hi\u1ec7u \u1ee9ng matrix) c\u0169ng nh\u01b0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i th\u1ea5p.\u00a0Ly t\u00e2m gradient m\u1eadt \u0111\u1ed9 l\u00e0 thu\u1eadn l\u1ee3i v\u00ec n\u00f3 cho ph\u00e9p ng\u01b0\u1eddi d\u00f9ng \u0111i\u1ec1u ch\u1ec9nh c\u00e1c th\u00f4ng s\u1ed1 \u0111\u1ec3 ph\u00e2n t\u00e1ch hi\u1ec7u qu\u1ea3 tu\u00e2n theo c\u00e1c \u0111\u1ecbnh lu\u1eadt nhi\u1ec7t \u0111\u1ed9ng l\u1ef1c h\u1ecdc, c\u00f3 th\u1ec3 s\u1eeda \u0111\u1ed5i \u0111\u1ed1i v\u1edbi t\u1ea5t c\u1ea3 c\u00e1c protein sau khi t\u1ed1i \u01b0u h\u00f3a th\u1eddi gian quay, t\u1ed1c \u0111\u1ed9 v\u00e0 \u0111i\u1ec1u ki\u1ec7n gradient.<\/p>\n
S\u1ef1 ti\u1ebfp x\u00fac oxy h\u00f3a c\u1ee7a protein \u1edf b\u1ec1 m\u1eb7t ch\u1ea5t l\u1ecfng kh\u00f4ng kh\u00ed c\u0169ng c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn s\u1ef1 gia t\u0103ng k\u1ebft t\u1ee5. C\u1ea7n c\u00f3 l\u01b0\u1ee3ng l\u1edbn ch\u1ea5t kh\u1eed trong dung d\u1ecbch \u0111\u1ec7m \u0111\u1ec3 t\u00e1ch SEC, \u0111i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 l\u00e0m t\u0103ng th\u00eam chi ph\u00ed cho th\u00ed nghi\u1ec7m.\u00a0H\u01a1n n\u1eefa, dung d\u1ecbch \u0111\u1ec7m \u0111i\u1ec3n h\u00ecnh \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n trong c\u00e1c chai ch\u1ee9a dung t\u00edch l\u1edbn, v\u1edbi di\u1ec7n t\u00edch b\u1ec1 m\u1eb7t kh\u00f4ng kh\u00ed-l\u1ecfng ng\u00e0y c\u00e0ng t\u0103ng.\u00a0Vi\u1ec7c b\u1ecbt k\u00edn c\u00e1c \u1ed1ng Opti-Seal v\u00e0 Quick-Seal t\u01b0\u01a1ng th\u00edch v\u1edbi m\u00e1y si\u00eau ly t\u00e2m<\/a> Beckman Coulter gi\u00fap gi\u1ea3m thi\u1ec3u b\u1ec1 m\u1eb7t ti\u1ebfp x\u00fac kh\u00f4ng kh\u00ed-l\u1ecfng, d\u1eabn \u0111\u1ebfn s\u1ea3n ph\u1ea9m protein \u1ed5n \u0111\u1ecbnh h\u01a1n v\u00e0 gi\u1ea3m s\u1ef1 k\u1ebft t\u1ee5.\u00a0H\u01a1n n\u1eefa, qu\u00e1 tr\u00ecnh ly t\u00e2m gi\u00fap lo\u1ea1i b\u1ecf nhu c\u1ea7u s\u1eed d\u1ee5ng th\u1ebb nhi\u1ec7t h\u1ea1ch, nh\u01b0\u1ee3c \u0111i\u1ec3m ph\u1ed5 bi\u1ebfn c\u1ee7a c\u00e1c k\u1ef9 thu\u1eadt tinh ch\u1ebf protein d\u1ef1a tr\u00ean s\u1eafc k\u00fd, c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u00e1ng k\u1ec3 \u0111\u1ebfn kh\u1ea3 n\u0103ng h\u00f2a tan protein.<\/p>\n
<\/span>Si\u00eau ly t\u00e2m ph\u00e2n t\u00edch<\/a> – M\u1ed9t c\u00f4ng c\u1ee5 m\u1ea1nh m\u1ebd \u0111\u1ec3 ph\u00e2n t\u00edch protein t\u1eeb c\u00e1c t\u1eadp h\u1ee3p<\/span><\/h2>\n
Nh\u01b0 \u0111\u00e3 th\u1ea3o lu\u1eadn \u1edf tr\u00ean, SEC l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 ph\u00e2n t\u00e1ch c\u00f3 nh\u1eefng sai s\u00f3t l\u1edbn, bao g\u1ed3m c\u00e1c hi\u1ec7u \u1ee9ng pha lo\u00e3ng v\u00e0 matrix \u0111\u00e1ng k\u1ec3. Do \u0111\u00f3, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y kh\u00f4ng l\u00fd t\u01b0\u1edfng cho vi\u1ec7c \u0111\u00e1nh gi\u00e1 quan tr\u1ecdng v\u1ec1 k\u00edch th\u01b0\u1edbc h\u1ea1t v\u00e0 s\u1ef1 k\u1ebft t\u1ee5 c\u1ee7a c\u00e1c s\u1ea3n ph\u1ea9m d\u1ef1a tr\u00ean protein. H\u01a1n n\u1eefa, n\u00f3 d\u1ef1a v\u00e0o c\u00e1c ti\u00eau chu\u1ea9n \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed, \u0111\u01b0a ra m\u1ed9t bi\u1ebfn b\u1ed5 sung l\u00e0m t\u0103ng bi\u00ean \u0111\u1ed9 sai s\u00f3t v\u00e0 kh\u1ea3 n\u0103ng kh\u00f4ng \u0111\u00e1ng tin c\u1eady c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p SEC.<\/p>\n
Ng\u01b0\u1ee3c l\u1ea1i, Si\u00eau ly t\u00e2m ph\u00e2n t\u00edch (AUC)<\/a> cung c\u1ea5p cho ng\u01b0\u1eddi d\u00f9ng nhi\u1ec1u c\u00e2u tr\u1ea3 l\u1eddi h\u01a1n b\u1ea5t k\u1ef3 k\u1ef9 thu\u1eadt t\u01b0\u01a1ng \u0111\u01b0\u01a1ng n\u00e0o. AUC<\/a> l\u00e0 thi\u1ebft b\u1ecb ch\u00ednh x\u00e1c v\u00e0 m\u1ea1nh m\u1ebd nh\u1ea5t \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng s\u1ef1 t\u1ed5ng h\u1ee3p protein. \u0110\u00e2y l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p tuy\u1ec7t \u0111\u1ed1i d\u1ef1a tr\u00ean m\u00f4 h\u00ecnh v\u1eadt l\u00fd nguy\u00ean l\u00fd \u0111\u1ea7u ti\u00ean \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh h\u00ecnh d\u1ea1ng ph\u00e2n t\u1eed, tr\u1ecdng l\u01b0\u1ee3ng v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh ph\u1ee9c t\u1ea1p \u1edf \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng nhi\u1ec1u c\u1ee1 m\u1eabu v\u00e0 n\u1ed3ng \u0111\u1ed9 kh\u00f4ng pha lo\u00e3ng. H\u01a1n n\u1eefa, s\u1ef1 t\u01b0\u01a1ng t\u00e1c b\u1ec1 m\u1eb7t t\u1ed1i thi\u1ec3u kh\u00f4ng c\u00f3 ti\u00eau chu\u1ea9n v\u00e0 kh\u00f4ng c\u00f3 ma tr\u1eadn mang \u0111\u1ebfn cho ng\u01b0\u1eddi d\u00f9ng kh\u1ea3 n\u0103ng s\u1eed d\u1ee5ng nhi\u1ec1u th\u00e0nh ph\u1ea7n \u0111\u1ec7m c\u00f3 th\u1ec3 c\u00f3.<\/p>\n
Trong nghi\u00ean c\u1ee9u c\u00e1c s\u1ea3n ph\u1ea9m protein v\u00e0 s\u1ef1 k\u1ebft t\u1ee5, th\u00e0nh ph\u1ea7n \u0111\u1ec7m (pH, c\u01b0\u1eddng \u0111\u1ed9 ion, ch\u1ea5t ph\u1ee5 gia, v.v.) l\u00e0 quan tr\u1ecdng nh\u1ea5t v\u00ec n\u00f3 \u1ea3nh h\u01b0\u1edfng tr\u1ef1c ti\u1ebfp \u0111\u1ebfn s\u1ef1 \u1ed5n \u0111\u1ecbnh c\u1ee7a protein. AUC h\u1ed7 tr\u1ee3 vi\u1ec7c l\u1ef1a ch\u1ecdn \u1ee9ng c\u1eed vi\u00ean thu\u1ed1c ch\u00ec, ph\u00e1t tri\u1ec3n c\u00f4ng th\u1ee9c sinh h\u1ecdc tr\u1ecb li\u1ec7u, m\u00f4 t\u1ea3 \u0111\u1eb7c t\u00ednh s\u1ea3n ph\u1ea9m v\u00e0 \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh. Vi\u1ec7c s\u1eed d\u1ee5ng AUC<\/a> trong ph\u00e2n t\u00edch ch\u1ea5t l\u01b0\u1ee3ng s\u1ea3n ph\u1ea9m c\u1ee7a c\u00e1c c\u00f4ng ty d\u01b0\u1ee3c ph\u1ea9m sinh h\u1ecdc c\u00f3 th\u1ec3 gi\u00fap x\u00e1c \u0111\u1ecbnh hi\u1ec7u qu\u1ea3 ph\u1ea3n \u1ee9ng mi\u1ec5n d\u1ecbch b\u1ea5t l\u1ee3i c\u00f3 th\u1ec3 c\u00f3 c\u1ee7a thu\u1ed1c d\u1ef1a tr\u00ean protein ho\u1eb7c thu\u1ed1c sinh h\u1ecdc t\u01b0\u01a1ng t\u1ef1.\u00a0 Optima AUC<\/a> m\u1edbi m\u1edf r\u1ed9ng ph\u1ea1m vi gi\u1ea3i ph\u00e1p do AUC<\/a> cung c\u1ea5p v\u00e0 cung c\u1ea5p cho ng\u01b0\u1eddi d\u00f9ng c\u00e1c th\u00f4ng s\u1ed1 l\u1eafng \u0111\u1ecdng ph\u00e2n t\u1eed v\u00e0 \u0111\u1eb7c t\u00ednh t\u1ed5ng h\u1ee3p \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh r\u00f5 r\u00e0ng.<\/p>\n
Ngu\u1ed3n: https:\/\/www.mybeckman.vn\/resources\/reading-material\/whitepapers\/centrifugation-workflow-solution-for-protein-purification-and-protein-aggregation-quantification<\/a><\/em><\/p>\n
Minh Khang l\u00e0 nh\u00e0 ph\u00e2n ph\u1ed1i v\u00e0 nh\u1eadp kh\u1ea9u tr\u1ef1c ti\u1ebfp c\u00e1c thi\u1ebft b\u1ecb ly t\u00e2m<\/a> h\u00e3ng Beckman Coulter<\/a>.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"
Ly t\u00e2m l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 m\u1ea1nh m\u1ebd cho quy tr\u00ecnh c\u00f4ng vi\u1ec7c \u0111\u1ec3 c\u00f4 l\u1eadp v\u00e0 x\u00e1c \u0111\u1ecbnh \u0111\u1eb7c t\u00ednh c\u1ee7a c\u00e1c s\u1ea3n ph\u1ea9m protein. S\u1ef1 t\u1eadp h\u1ee3p protein l\u00e0 m\u1ed9t hi\u1ec7n t\u01b0\u1ee3ng sinh h\u1ecdc g\u00e2y ra b\u1edfi s\u1ef1 t\u01b0\u01a1ng t\u00e1c b\u1ea5t th\u01b0\u1eddng c\u1ee7a c\u00e1c protein b\u1ecb m\u1ea5t \u1ed5n \u0111\u1ecbnh. \u0110\u1eb7c t\u00ednh ho\u1ea1t \u0111\u1ed9ng…<\/p>\n","protected":false},"author":3,"featured_media":10132,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"rank_math_lock_modified_date":false,"footnotes":""},"categories":[40],"tags":[],"class_list":["post-10129","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"acf":[],"_links":{"self":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10129"}],"collection":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/comments?post=10129"}],"version-history":[{"count":3,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10129\/revisions"}],"predecessor-version":[{"id":10133,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10129\/revisions\/10133"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media\/10132"}],"wp:attachment":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media?parent=10129"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/categories?post=10129"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/tags?post=10129"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}