{"id":10339,"date":"2023-11-30T07:54:26","date_gmt":"2023-11-30T07:54:26","guid":{"rendered":"https:\/\/gd-1.com\/?p=10339"},"modified":"2023-11-30T08:07:27","modified_gmt":"2023-11-30T08:07:27","slug":"nguon-sang-kinh-hien-vi-nguyen-tac-co-ban-cua-den-ho-quang-thuy-ngan","status":"publish","type":"post","link":"https:\/\/gd-1.com\/nguon-sang-kinh-hien-vi-nguyen-tac-co-ban-cua-den-ho-quang-thuy-ngan\/","title":{"rendered":"Ngu\u1ed3n s\u00e1ng k\u00ednh hi\u1ec3n vi &#8211; Nguy\u00ean t\u1eafc c\u01a1 b\u1ea3n c\u1ee7a \u0111\u00e8n h\u1ed3 quang th\u1ee7y ng\u00e2n"},"content":{"rendered":"<h2 class=\"paratitle\"><strong>M\u1ee9c c\u00f4ng su\u1ea5t ngu\u1ed3n s\u00e1ng <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi<\/a><\/strong><\/h2>\n<p>Vi\u1ec7c ch\u1ecdn ngu\u1ed3n s\u00e1ng th\u00edch h\u1ee3p \u0111\u1ec3 nghi\u00ean c\u1ee9u b\u1eb1ng <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi hu\u1ef3nh quang<\/a> ph\u1ee5 thu\u1ed9c nhi\u1ec1u v\u00e0o ph\u01b0\u01a1ng ph\u00e1p chi\u1ebfu s\u00e1ng (truy\u1ec1n qua ho\u1eb7c tr\u1ef1c quan), th\u00f4ng s\u1ed1 m\u1eabu, c\u1ea5u h\u00ecnh <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi<\/a> v\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ee7a m\u00e1y d\u00f2. Trong <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi hu\u1ef3nh quang<\/a>, lo\u1ea1i fluorophore (t\u1ed5ng h\u1ee3p, protein hu\u1ef3nh quang, ch\u1ea5m l\u01b0\u1ee3ng t\u1eed, v.v.), \u0111\u1eb7c \u0111i\u1ec3m c\u1ee7a b\u1ed9 l\u1ecdc (b\u0103ng th\u00f4ng v\u00e0 c\u1ea5u h\u00ecnh) v\u00e0 t\u1ed1c \u0111\u1ed9 thu c\u1ee7a m\u00e1y d\u00f2 l\u00e0 c\u00e1c bi\u1ebfn s\u1ed1 c\u0169ng ph\u1ea3i \u0111\u01b0\u1ee3c xem x\u00e9t. Theo nguy\u00ean t\u1eafc chung, fluorophores t\u1ed5ng h\u1ee3p v\u00e0 ch\u1ea5m l\u01b0\u1ee3ng t\u1eed c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u1edbi c\u00e1c t\u1ebf b\u00e0o c\u1ed1 \u0111\u1ecbnh c\u00f3 c\u00f4ng su\u1ea5t chi\u1ebfu s\u00e1ng cao, trong khi \u0111\u00f3 khi ch\u1ee5p \u1ea3nh t\u1ebf b\u00e0o s\u1ed1ng, protein hu\u1ef3nh quang v\u00e0 c\u00e1c \u0111\u1ea7u d\u00f2 kh\u00e1c ph\u1ea3i \u0111\u01b0\u1ee3c k\u00edch th\u00edch \u1edf m\u1ee9c n\u0103ng l\u01b0\u1ee3ng th\u1ea5p h\u01a1n nhi\u1ec1u. C\u00e1c b\u1ea3ng \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y d\u01b0\u1edbi \u0111\u00e2y l\u00e0 s\u1ef1 t\u1ed5ng h\u1ee3p c\u00e1c c\u00f4ng su\u1ea5t \u0111\u1ea7u ra so s\u00e1nh (\u0111\u01b0\u1ee3c \u0111o \u1edf m\u1eb7t ph\u1eb3ng ti\u00eau c\u1ef1 v\u1eadt k\u00ednh) \u0111\u1ed1i v\u1edbi c\u00e1c ngu\u1ed3n s\u00e1ng kh\u00f4ng k\u1ebft h\u1ee3p ph\u1ed5 bi\u1ebfn nh\u1ea5t \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong<a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\"> k\u00ednh hi\u1ec3n vi hu\u1ef3nh quang<\/a> epi.<\/p>\n<p style=\"text-align: center;\"><strong>B\u1ea3ng 1: C\u00f4ng su\u1ea5t quang h\u1ecdc so s\u00e1nh c\u1ee7a c\u00e1c ngu\u1ed3n s\u00e1ng<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-10342 aligncenter\" src=\"https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.51.png\" alt=\"\" width=\"711\" height=\"290\" srcset=\"https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.51.png 1324w, https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.51-300x122.png 300w, https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.51-1024x418.png 1024w, https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.51-768x313.png 768w, https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.51-510x208.png 510w\" sizes=\"(max-width: 711px) 100vw, 711px\" \/><\/p>\n<p>C\u00f4ng su\u1ea5t quang \u0111\u1ea7u ra (\u0111\u01b0\u1ee3c bi\u1ec3u th\u1ecb b\u1eb1ng miliwatt\/cm<sup>2<\/sup>) c\u1ee7a c\u00e1c ngu\u1ed3n s\u00e1ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t trong <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi hu\u1ef3nh quang<\/a> tr\u01b0\u1eddng r\u1ed9ng \u0111\u01b0\u1ee3c so s\u00e1nh trong <em>B\u1ea3ng 1.<\/em> C\u00e1c m\u1ee9c c\u00f4ng su\u1ea5t \u0111\u01b0\u1ee3c \u0111o t\u1ea1i m\u1eb7t ph\u1eb3ng ti\u00eau c\u1ef1 c\u1ee7a v\u1eadt <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi<\/a> (v\u1eadt k\u00ednh kh\u00f4 40x fluorit, kh\u1ea9u \u0111\u1ed9 s\u1ed1 = 0,85 ) b\u1eb1ng m\u00e1y \u0111o b\u1ee9c x\u1ea1 d\u1ef1a tr\u00ean photodiode.\u00a0Ngu\u1ed3n s\u00e1ng \u0111\u01b0\u1ee3c gh\u00e9p tr\u1ef1c ti\u1ebfp v\u1edbi h\u1ec7 th\u1ed1ng quang h\u1ecdc chi\u1ebfu s\u00e1ng tr\u1ef1c ti\u1ebfp c\u1ee7a <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi<\/a> (ngu\u1ed3n xenon, th\u1ee7y ng\u00e2n, vonfram-halogen v\u00e0 \u0111\u00e8n LED) ho\u1eb7c \u0111\u01b0\u1ee3c d\u1eabn qua b\u1ed9 d\u1eabn \u00e1nh s\u00e1ng l\u1ecfng v\u00e0 th\u1ea5u k\u00ednh chu\u1ea9n tr\u1ef1c g\u1eafn v\u00e0o \u0111\u00e8n chi\u1ebfu s\u00e1ng (ngu\u1ed3n halogen kim lo\u1ea1i).\u00a0\u00c1nh s\u00e1ng t\u1eeb ngu\u1ed3n \u0111\u01b0\u1ee3c truy\u1ec1n qua d\u00e3y quang h\u1ecdc c\u1ee7a <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi<\/a> v\u00e0 v\u00e0o c\u00e1c b\u1ed9 l\u1ecdc hu\u1ef3nh quang \u0111\u00e3 ch\u1ecdn \u0111\u01b0\u1ee3c li\u1ec7t k\u00ea trong c\u1ed9t \u0111\u1ea7u ti\u00ean c\u1ee7a <em>B\u1ea3ng 1.<\/em> C\u00e1c ph\u00e9p \u0111o c\u00f4ng su\u1ea5t bi\u1ec3u th\u1ecb l\u01b0\u1ee3ng \u00e1nh s\u00e1ng \u0111i qua b\u1ed9 l\u1ecdc k\u00edch th\u00edch v\u00e0 \u0111\u01b0\u1ee3c ph\u1ea3n x\u1ea1 v\u00e0o v\u1eadt k\u00ednh b\u1eb1ng g\u01b0\u01a1ng l\u01b0\u1ee1ng s\u1eafc cho m\u1ed7i b\u1ed9 l\u1ecdc.\u00a0L\u01b0u \u00fd r\u1eb1ng ngu\u1ed3n s\u00e1ng vonfram-halogen, c\u00f3 c\u00f4ng su\u1ea5t k\u00edch th\u00edch th\u1ea5p nh\u1ea5t so v\u1edbi b\u1ea5t k\u1ef3 ngu\u1ed3n n\u00e0o \u0111\u01b0\u1ee3c li\u1ec7t k\u00ea trong b\u1ea3ng, \u0111\u01b0\u1ee3c v\u1eadn h\u00e0nh \u1edf \u0111i\u1ec7n \u00e1p cao nh\u1ea5t c\u00f3 th\u1ec3 cho c\u00e1c ph\u00e9p \u0111o n\u00e0y.<\/p>\n<p style=\"text-align: center;\"><strong>B\u1ea3ng 2: C\u00f4ng su\u1ea5t quang h\u1ecdc so s\u00e1nh c\u1ee7a c\u00e1c ngu\u1ed3n s\u00e1ng halogen kim lo\u1ea1i<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-10341 aligncenter\" src=\"https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.19.png\" alt=\"\" width=\"710\" height=\"337\" srcset=\"https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.19.png 1132w, https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.19-300x143.png 300w, https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.19-1024x487.png 1024w, https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.19-768x365.png 768w, https:\/\/gd-1.com\/wp-content\/uploads\/2023\/11\/Anh-man-hinh-2023-11-30-luc-11.09.19-510x242.png 510w\" sizes=\"(max-width: 710px) 100vw, 710px\" \/><\/p>\n<p>\u0110\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong <em>B\u1ea3ng 2<\/em> l\u00e0 c\u00e1c gi\u00e1 tr\u1ecb c\u00f4ng su\u1ea5t quang \u0111\u1ea7u ra c\u1ee7a m\u1ed9t s\u1ed1 ngu\u1ed3n s\u00e1ng halogen kim lo\u1ea1i sau khi truy\u1ec1n t\u1eeb v\u1eadt d\u1eabn \u00e1nh s\u00e1ng l\u1ecfng qua d\u00e3y quang h\u1ecdc c\u1ee7a <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi<\/a> v\u00e0 c\u00e1c b\u1ed9 l\u1ecdc hu\u1ef3nh quang \u0111\u01b0\u1ee3c ch\u1ecdn. C\u00f4ng su\u1ea5t (t\u00ednh b\u1eb1ng miliwatt\/cm2) \u0111\u01b0\u1ee3c \u0111o t\u1ea1i m\u1eb7t ph\u1eb3ng ti\u00eau \u0111i\u1ec3m c\u1ee7a v\u1eadt k\u00ednh hi\u1ec3n vi (v\u1eadt k\u00ednh kh\u00f4 40x fluorite, kh\u1ea9u \u0111\u1ed9 s\u1ed1 = 0,85) b\u1eb1ng m\u00e1y \u0111o ph\u00f3ng x\u1ea1 d\u1ef1a tr\u00ean photodiode. M\u1ed9t chi\u1ebfc g\u01b0\u01a1ng c\u00f3 \u0111\u1ed9 ph\u1ea3n x\u1ea1 l\u1edbn h\u01a1n 95% t\u1eeb 350 \u0111\u1ebfn 800 nanomet ho\u1eb7c b\u1ed9 l\u1ecdc hu\u1ef3nh quang ti\u00eau chu\u1ea9n \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 chi\u1ebfu \u00e1nh s\u00e1ng qua v\u1eadt k\u00ednh v\u00e0 v\u00e0o c\u1ea3m bi\u1ebfn \u0111o b\u1ee9c x\u1ea1. \u00c1nh s\u00e1ng truy\u1ec1n ra c\u00f3 th\u1ec3 thay \u0111\u1ed5i trong kho\u1ea3ng t\u1eeb 50 \u0111\u1ebfn 99 ph\u1ea7n tr\u0103m c\u00f4ng su\u1ea5t \u0111\u1ea7u v\u00e0o, t\u00f9y thu\u1ed9c v\u00e0o c\u01a1 ch\u1ebf gh\u00e9p ngu\u1ed3n s\u00e1ng v\u00e0 s\u1ed1 l\u01b0\u1ee3ng b\u1ed9 l\u1ecdc, g\u01b0\u01a1ng, l\u0103ng k\u00ednh v\u00e0 th\u1ea5u k\u00ednh trong d\u00e3y quang h\u1ecdc. V\u00ed d\u1ee5, \u0111\u1ed1i v\u1edbi m\u1ed9t <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi<\/a> soi ng\u01b0\u1ee3c c\u1ea5p \u0111\u1ed9 nghi\u00ean c\u1ee9u \u0111i\u1ec3n h\u00ecnh \u0111\u01b0\u1ee3c gh\u00e9p n\u1ed1i v\u1edbi ngu\u1ed3n chi\u1ebfu s\u00e1ng halogen kim lo\u1ea1i b\u00ean ngo\u00e0i, c\u00f3 \u00edt h\u01a1n 20 ph\u1ea7n tr\u0103m \u00e1nh s\u00e1ng tho\u00e1t ra kh\u1ecfi \u1ed1ng d\u1eabn \u00e1nh s\u00e1ng l\u1ecfng \u1edf l\u1ed1i v\u00e0o c\u1ee7a h\u1ec7 th\u1ed1ng th\u1ea5u k\u00ednh chu\u1ea9n tr\u1ef1c c\u00f3 s\u1eb5n \u0111\u1ec3 k\u00edch th\u00edch c\u00e1c hu\u1ef3nh quang \u0111\u1eb7t \u1edf ph\u00eda tr\u01b0\u1edbc m\u1eb7t ph\u1eb3ng ti\u00eau c\u1ef1. Ph\u1ea1m vi \u00e1nh s\u00e1ng truy\u1ec1n ra t\u01b0\u01a1ng t\u1ef1 x\u1ea3y ra v\u1edbi \u0110\u00e8n h\u1ed3 quang\u00a0ng\u1eafn\u00a0xenon v\u00e0 \u0111\u00e8n th\u1ee7y ng\u00e2n \u0111\u01b0\u1ee3c g\u1eafn tr\u1ef1c ti\u1ebfp v\u00e0o ngu\u1ed3n s\u00e1ng th\u00f4ng qua h\u1ec7 th\u1ed1ng \u0111\u00e8n.<\/p>\n<p><em>Ngu\u1ed3n: <a href=\"https:\/\/zeiss-campus.magnet.fsu.edu\/articles\/lightsources\/powertable.html\" target=\"_blank\" rel=\"noopener\">https:\/\/zeiss-campus.magnet.fsu.edu\/articles\/lightsources\/powertable.html<\/a><\/em><\/p>\n<p><strong>C\u00f4ng ty Minh Khang l\u00e0 nh\u00e0 ph\u00e2n ph\u1ed1i \u0111\u1ed9c quy\u1ec1n th\u1ecb tr\u01b0\u1eddng mi\u1ec1n Nam ph\u00e2n kh\u00fac <a href=\"https:\/\/gd-1.com\/product-category\/kinh-hien-vi\/\">k\u00ednh hi\u1ec3n vi<\/a> h\u00e3ng\u00a0<a href=\"https:\/\/gd-1.com\/product-brands\/zeiss-vi\/\">Carl ZEISS<\/a>.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>M\u1ee9c c\u00f4ng su\u1ea5t ngu\u1ed3n s\u00e1ng k\u00ednh hi\u1ec3n vi Vi\u1ec7c ch\u1ecdn ngu\u1ed3n s\u00e1ng th\u00edch h\u1ee3p \u0111\u1ec3 nghi\u00ean c\u1ee9u b\u1eb1ng k\u00ednh hi\u1ec3n vi hu\u1ef3nh quang ph\u1ee5 thu\u1ed9c nhi\u1ec1u v\u00e0o ph\u01b0\u01a1ng ph\u00e1p chi\u1ebfu s\u00e1ng (truy\u1ec1n qua ho\u1eb7c tr\u1ef1c quan), th\u00f4ng s\u1ed1 m\u1eabu, c\u1ea5u h\u00ecnh k\u00ednh hi\u1ec3n vi v\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ee7a m\u00e1y d\u00f2. Trong k\u00ednh hi\u1ec3n vi hu\u1ef3nh&#8230;<\/p>\n","protected":false},"author":3,"featured_media":10343,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[40],"tags":[],"_links":{"self":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10339"}],"collection":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/comments?post=10339"}],"version-history":[{"count":3,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10339\/revisions"}],"predecessor-version":[{"id":10350,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/10339\/revisions\/10350"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media\/10343"}],"wp:attachment":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media?parent=10339"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/categories?post=10339"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/tags?post=10339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}