{"id":8043,"date":"2023-05-15T02:55:15","date_gmt":"2023-05-15T02:55:15","guid":{"rendered":"https:\/\/gd-1.com\/?p=8043"},"modified":"2023-05-17T06:09:30","modified_gmt":"2023-05-17T06:09:30","slug":"automated-1-hour-mycoplasma-testing-in-bioproduction","status":"publish","type":"post","link":"https:\/\/gd-1.com\/en\/automated-1-hour-mycoplasma-testing-in-bioproduction\/","title":{"rendered":"Automated 1-Hour Mycoplasma Testing in Bioproduction"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_69_1 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<label class=\"ez-toc-title ez-toc-toggle\" style=\"cursor:pointer\">T\u00f3m t\u1eaft n\u1ed9i dung<\/label>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 eztoc-toggle-hide-by-default' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/gd-1.com\/en\/automated-1-hour-mycoplasma-testing-in-bioproduction\/#INTRODUCTION\" title=\"INTRODUCTION\">INTRODUCTION<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/gd-1.com\/en\/automated-1-hour-mycoplasma-testing-in-bioproduction\/#THE_VALUE_OF_RAPID_EASY_MYCOPLASMA_TESTING\" title=\"THE VALUE OF RAPID, EASY MYCOPLASMA TESTING\">THE VALUE OF RAPID, EASY MYCOPLASMA TESTING<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/gd-1.com\/en\/automated-1-hour-mycoplasma-testing-in-bioproduction\/#BIOFIRE_FILMARRAY_20_INDUSTRY_SYSTEM\" title=\"BIOFIRE FILMARRAY 2.0 INDUSTRY SYSTEM\">BIOFIRE FILMARRAY 2.0 INDUSTRY SYSTEM<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/gd-1.com\/en\/automated-1-hour-mycoplasma-testing-in-bioproduction\/#SAMPLE_PROTOCOLS\" title=\"SAMPLE PROTOCOLS\">SAMPLE PROTOCOLS<\/a><\/li><\/ul><\/nav><\/div>\n<div class=\"text aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"text-3edeff4e75\" class=\"cmp-text\" style=\"box-sizing: border-box; font-size: var(--font-text-rg); position: relative; z-index: 1;\" data-cmp-data-layer=\"{&quot;text-3edeff4e75&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/text&quot;,&quot;repo:modifyDate&quot;:&quot;2023-01-26T09:39:51Z&quot;,&quot;xdm:text&quot;:&quot;&lt;h2&gt;INTRODUCTION&lt;\/h2&gt;\\r\\n&lt;p&gt;Various pharmacopoeias, including the USP, specify a test for mycoplasma\u00a0 contamination must be\u00a0performed as part of the release testing of a product manufactured in the presence of eukaryotic cells\u00a0&lt;sup&gt;(1)&lt;\/sup&gt;.&lt;br \/&gt;\\r\\nCurrent compendial methods require \u226528 days to generate results creating delays\u00a0 releasing product\u00a0to downstream processes. Alternative conventional nucleic acid testing (NAT) methods are available;\u00a0however, until recently these methods provided speed (~5 h) but not ease of use.&lt;\/p&gt;\\r\\n&lt;p&gt;The BIOFIRE&lt;sup&gt;\u00ae&lt;\/sup&gt;\u00a0FILMARRAY&lt;sup&gt;\u00ae&lt;\/sup&gt;\u00a02.0 industry system utilizes a film array instrument and next generation\u00a0PCR testing where all reagent components are contained in a closed pouch to detect the presence of\u00a0&amp;gt;130 species of mycoplasma. The system provides sample to answer in ~1 hour with little technical\u00a0training\u00a0 needed providing options for bringing mycoplasma testing directly to the production floor where\u00a0critical testing is needed.&lt;\/p&gt;\\r\\n&quot;}}\">\n<h2><span class=\"ez-toc-section\" id=\"INTRODUCTION\"><\/span>INTRODUCTION<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Various pharmacopoeias, including the USP, specify a test for mycoplasma\u00a0 contamination must be\u00a0performed as part of the release testing of a product manufactured in the presence of eukaryotic cells\u00a0<sup>(1)<\/sup>.<br \/>\nCurrent compendial methods require \u226528 days to generate results creating delays\u00a0 releasing product\u00a0to downstream processes. Alternative conventional nucleic acid testing (NAT) methods are available;\u00a0however, until recently these methods provided speed (~5 h) but not ease of use.<\/p>\n<p>The BIOFIRE<sup>\u00ae<\/sup>\u00a0FILMARRAY<sup>\u00ae<\/sup>\u00a02.0 industry system utilizes a film array instrument and next generation\u00a0PCR testing where all reagent components are contained in a closed pouch to detect the presence of\u00a0&gt;130 species of mycoplasma. The system provides sample to answer in ~1 hour with little technical\u00a0training\u00a0 needed providing options for bringing mycoplasma testing directly to the production floor where\u00a0critical testing is needed.<\/p>\n<\/div>\n<\/div>\n<div class=\"image aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"image-107b380ac7\" class=\"cmp-image\" data-cmp-lazy=\"\" data-cmp-lazythreshold=\"0\" data-cmp-src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/BIOFIRE%20MYCOPLASMA%20TESTING%20METHOD_0?qlt=85&amp;wid=%7B.width%7D&amp;ts=1668105518627&amp;dpr=off\" data-cmp-widths=\"320,480,600,800,1024,1200,1600\" data-cmp-dmimage=\"\" data-asset=\"\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/BIOFIRE MYCOPLASMA TESTING METHOD_0.png\" data-title=\"BIREFIRE testing chart\" data-cmp-data-layer=\"{&quot;image-107b380ac7&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/image&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:38:17Z&quot;,&quot;image&quot;:{&quot;repo:id&quot;:&quot;9eb6b085-3f44-4175-ab54-708bc45be60e&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:38:38Z&quot;,&quot;@type&quot;:&quot;image\/png&quot;,&quot;repo:path&quot;:&quot;\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/BIOFIRE MYCOPLASMA TESTING METHOD_0.png&quot;,&quot;xdm:smartTags&quot;:{&quot;template&quot;:0.6969557404518127,&quot;symbol&quot;:0.6572567224502563,&quot;icon&quot;:0.7374213933944702,&quot;sign&quot;:0.652452826499939,&quot;interface&quot;:0.6323864459991455,&quot;button&quot;:0.7135125398635864,&quot;bar&quot;:0.5924078226089478,&quot;modern&quot;:0.6008903384208679,&quot;web&quot;:0.7442193627357483,&quot;design&quot;:0.755596935749054,&quot;vector&quot;:0.7757628560066223,&quot;element&quot;:0.6444177627563477,&quot;calendar&quot;:0.6492984294891357,&quot;website&quot;:0.6711368560791016,&quot;set&quot;:0.6277334094047546,&quot;business&quot;:0.7178345918655396,&quot;banner&quot;:0.5932044386863708,&quot;menu&quot;:0.6071397662162781,&quot;layout&quot;:0.640783965587616,&quot;site&quot;:0.5973060131072998,&quot;blue&quot;:0.6699888706207275,&quot;background&quot;:0.6436768174171448,&quot;illustration&quot;:0.6733337640762329,&quot;page&quot;:0.6485816836357117,&quot;internet&quot;:0.641639232635498}}}}\"><img decoding=\"async\" class=\"cmp-image__image\" src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/BIOFIRE%20MYCOPLASMA%20TESTING%20METHOD_0?qlt=85&amp;wid=1600&amp;ts=1668105518627&amp;dpr=off\" alt=\"BIREFIRE testing chart\" data-cmp-hook-image=\"image\" \/><\/div>\n<\/div>\n<div class=\"text aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"text-17f6162711\" class=\"cmp-text\" style=\"box-sizing: border-box; font-size: var(--font-text-rg); position: relative; z-index: 1;\" data-cmp-data-layer=\"{&quot;text-17f6162711&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/text&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T19:02:48Z&quot;,&quot;xdm:text&quot;:&quot;&lt;p style=\\&quot;text-align: center;\\&quot;&gt;*10 mL protocol&lt;\/p&gt;\\r\\n&quot;}}\">\n<p>*10 mL protocol<\/p>\n<\/div>\n<\/div>\n<div class=\"text aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"text-60fd0cf67d\" class=\"cmp-text\" style=\"box-sizing: border-box; font-size: var(--font-text-rg); position: relative; z-index: 1;\" data-cmp-data-layer=\"{&quot;text-60fd0cf67d&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/text&quot;,&quot;repo:modifyDate&quot;:&quot;2023-01-26T15:24:41Z&quot;,&quot;xdm:text&quot;:&quot;&lt;p&gt;As presented at the 2021 PDA Pharmaceutical Microbiology Virtual Conference, the data summarized&amp;nbsp;in this White Paper is from four &lt;a title=\\&quot;bioproduction manufacturers\\&quot; href=\\&quot;\/content\/biomerieux\/corp\/en\/our-offer\/pharmaceutical-quality-control\/biopharmaceuticals.html\\&quot; target=\\&quot;_blank\\&quot;&gt;bioproduction manufacturers&lt;\/a&gt; that evaluated the BIOFIRE&lt;sup&gt;\u00ae&lt;\/sup&gt;FILMARRAY&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;2.0 Industry system&amp;nbsp;&lt;sup&gt;(2)&lt;\/sup&gt;. Samples were evaluated with up to 9 compendial mycoplasma species in the&amp;nbsp;presence of high-density monoclonal antibody producing Chinese hamster ovary (CHO) cells. Studies&amp;nbsp;were designed to assess product interference (false-positive rates), and detection (false-negative rates)&amp;nbsp;including level of detection (LOD). Two distinct protocols were evaluated: a protocol using 10 mL of&amp;nbsp;product sample that provides the sensitivity for release testing, and a direct-test protocol using 0.2 mL&amp;nbsp;product sample that allows for at-line in-process control testing.&lt;\/p&gt;\\r\\n&lt;h2&gt;&amp;nbsp;&lt;\/h2&gt;\\r\\n&lt;h2&gt;THE VALUE OF RAPID, EASY MYCOPLASMA TESTING&lt;\/h2&gt;\\r\\n&lt;p&gt;A faster, easier approach to mycoplasma testing can bring quantifiable value to bioproduction&amp;nbsp;manufacturers. From simplifying training requirements, to reducing investigations linked to human&amp;nbsp;error, to providing an early alert to contamination, an ultra rapid, ultra easy mycoplasma test can save&amp;nbsp;time and money bringing value to any bioproduction organization.&lt;br&gt;\\r\\nThe BIOFIRE&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;mycoplasma test can be performed by anyone, anywhere at anytime allowing manufacturers to take critical quality testing out of the lab and closer to manufacturing to realize the&amp;nbsp;following benefits:&lt;\/p&gt;\\r\\n&lt;p&gt;\u2022 Simplified training requirements&lt;br&gt;\\r\\n\u2022 Lower expertise required&lt;br&gt;\\r\\n\u2022 Objective results&lt;br&gt;\\r\\n\u2022 Less human error risk\/improved Data Integrity&lt;br&gt;\\r\\n\u2022 Does not require PCR lab&lt;br&gt;\\r\\n\u2022 1 hour time-to-result brings flexibility in testing planning&lt;br&gt;\\r\\n\u2022 Provides early alert to contamination and reduces costs of non-quality&lt;\/p&gt;\\r\\n&quot;}}\">\n<p>As presented at the 2021 PDA Pharmaceutical Microbiology Virtual Conference, the data summarized\u00a0in this White Paper is from four <a title=\"bioproduction manufacturers\" href=\"https:\/\/www.biomerieux.com\/corp\/en\/our-offer\/pharmaceutical-quality-control\/biopharmaceuticals.html\" target=\"_blank\" rel=\"noopener noreferrer\">bioproduction manufacturers<\/a> that evaluated the BIOFIRE<sup>\u00ae<\/sup>FILMARRAY<sup>\u00ae<\/sup>\u00a02.0 Industry system\u00a0<sup>(2)<\/sup>. Samples were evaluated with up to 9 compendial mycoplasma species in the\u00a0presence of high-density monoclonal antibody producing Chinese hamster ovary (CHO) cells. Studies\u00a0were designed to assess product interference (false-positive rates), and detection (false-negative rates)\u00a0including level of detection (LOD). Two distinct protocols were evaluated: a protocol using 10 mL of\u00a0product sample that provides the sensitivity for release testing, and a direct-test protocol using 0.2 mL\u00a0product sample that allows for at-line in-process control testing.<\/p>\n<h2><\/h2>\n<h2><span class=\"ez-toc-section\" id=\"THE_VALUE_OF_RAPID_EASY_MYCOPLASMA_TESTING\"><\/span>THE VALUE OF RAPID, EASY MYCOPLASMA TESTING<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>A faster, easier approach to mycoplasma testing can bring quantifiable value to bioproduction\u00a0manufacturers. From simplifying training requirements, to reducing investigations linked to human\u00a0error, to providing an early alert to contamination, an ultra rapid, ultra easy mycoplasma test can save\u00a0time and money bringing value to any bioproduction organization.<br \/>\nThe BIOFIRE<sup>\u00ae<\/sup>\u00a0mycoplasma test can be performed by anyone, anywhere at anytime allowing manufacturers to take critical quality testing out of the lab and closer to manufacturing to realize the\u00a0following benefits:<\/p>\n<p>\u2022 Simplified training requirements<br \/>\n\u2022 Lower expertise required<br \/>\n\u2022 Objective results<br \/>\n\u2022 Less human error risk\/improved Data Integrity<br \/>\n\u2022 Does not require PCR lab<br \/>\n\u2022 1 hour time-to-result brings flexibility in testing planning<br \/>\n\u2022 Provides early alert to contamination and reduces costs of non-quality<\/p>\n<\/div>\n<\/div>\n<div class=\"image aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"image-4500cf6f9a\" class=\"cmp-image\" data-cmp-lazy=\"\" data-cmp-lazythreshold=\"0\" data-cmp-src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/Anyone%20anywhere%20anytime?qlt=85&amp;wid=%7B.width%7D&amp;ts=1668105829386&amp;dpr=off\" data-cmp-widths=\"320,480,600,800,1024,1200,1600\" data-cmp-dmimage=\"\" data-asset=\"\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/Anyone anywhere anytime.jpeg\" data-title=\"anyone, anywhere, anytime\" data-cmp-data-layer=\"{&quot;image-4500cf6f9a&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/image&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:43:49Z&quot;,&quot;image&quot;:{&quot;repo:id&quot;:&quot;dd3f2305-5a4d-4629-a7a6-27cbfdd86fe4&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:41:35Z&quot;,&quot;@type&quot;:&quot;image\/jpeg&quot;,&quot;repo:path&quot;:&quot;\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/Anyone anywhere anytime.jpeg&quot;,&quot;xdm:smartTags&quot;:{&quot;symbol&quot;:0.6943519115447998,&quot;data&quot;:0.544296145439148,&quot;concept&quot;:0.6452863216400146,&quot;document&quot;:0.5976200699806213,&quot;icon&quot;:0.7983948588371277,&quot;sign&quot;:0.6634520292282104,&quot;isolated&quot;:0.5896766185760498,&quot;house&quot;:0.5633285045623779,&quot;network&quot;:0.6144528388977051,&quot;button&quot;:0.7101881504058838,&quot;computer&quot;:0.5839093923568726,&quot;security&quot;:0.5443102121353149,&quot;file&quot;:0.5403528809547424,&quot;white&quot;:0.5827016830444336,&quot;web&quot;:0.5601276159286499,&quot;design&quot;:0.6392145156860352,&quot;vector&quot;:0.6724346876144409,&quot;graphic&quot;:0.5418728590011597,&quot;business&quot;:0.6706074476242065,&quot;black&quot;:0.558941662311554,&quot;technology&quot;:0.5511497855186462,&quot;blue&quot;:0.673128068447113,&quot;illustration&quot;:0.6608885526657104,&quot;circle&quot;:0.5766957998275757,&quot;internet&quot;:0.5953249335289001}}}}\"><img decoding=\"async\" class=\"cmp-image__image\" src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/Anyone%20anywhere%20anytime?qlt=85&amp;wid=1600&amp;ts=1668105829386&amp;dpr=off\" alt=\"anyone, anywhere, anytime\" data-cmp-hook-image=\"image\" \/><\/div>\n<\/div>\n<div class=\"text aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"text-49d0d83d42\" class=\"cmp-text\" style=\"box-sizing: border-box; font-size: var(--font-text-rg); position: relative; z-index: 1;\" data-cmp-data-layer=\"{&quot;text-49d0d83d42&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/text&quot;,&quot;repo:modifyDate&quot;:&quot;2023-01-26T10:12:03Z&quot;,&quot;xdm:text&quot;:&quot;&lt;p&gt;Because BIOFIRE&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;FILMARRAY&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;2.0 is so easy to use, manufacturers who previously lacked the&amp;nbsp;resources to perform mycoplasma testing in-house can now easily perform mycoplasma screening&amp;nbsp;at-line with little specialized equipment or training saving on costly outsourced laboratory testing.&lt;\/p&gt;\\r\\n&lt;p&gt;&lt;br&gt;\\r\\nBioproduction manufacturers today waiting hours or weeks for results using complex testing&amp;nbsp;methods can realize significant value with a more rapid, simplified approach to &lt;a title=\\&quot;Automated Maycoplasma Testing\\&quot; href=\\&quot;\/content\/biomerieux\/corp\/en\/our-offer\/industry-products\/biofire-mycoplasma-mycoplasma-detection.html\\&quot; target=\\&quot;_blank\\&quot;&gt;mycoplasma testing&amp;nbsp;using BIOFIRE&lt;sup&gt;\u00ae&lt;\/sup&gt;FILMARRAY&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;2.0&lt;\/a&gt; Industry.&lt;\/p&gt;\\r\\n&quot;}}\">\n<p>Because BIOFIRE<sup>\u00ae<\/sup>\u00a0FILMARRAY<sup>\u00ae<\/sup>\u00a02.0 is so easy to use, manufacturers who previously lacked the\u00a0resources to perform mycoplasma testing in-house can now easily perform mycoplasma screening\u00a0at-line with little specialized equipment or training saving on costly outsourced laboratory testing.<\/p>\n<p>Bioproduction manufacturers today waiting hours or weeks for results using complex testing\u00a0methods can realize significant value with a more rapid, simplified approach to <a title=\"Automated Maycoplasma Testing\" href=\"https:\/\/www.biomerieux.com\/corp\/en\/our-offer\/industry-products\/biofire-mycoplasma-mycoplasma-detection.html\" target=\"_blank\" rel=\"noopener noreferrer\">mycoplasma testing\u00a0using BIOFIRE<sup>\u00ae<\/sup>FILMARRAY<sup>\u00ae<\/sup>\u00a02.0<\/a> Industry.<\/p>\n<\/div>\n<\/div>\n<div class=\"image aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"image-e22390c7e8\" class=\"cmp-image\" data-cmp-lazy=\"\" data-cmp-lazythreshold=\"0\" data-cmp-src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/BIOFIRE%20MYCOPLASMA%20PROTOCOL?qlt=85&amp;wid=%7B.width%7D&amp;ts=1668105885165&amp;dpr=off\" data-cmp-widths=\"320,480,600,800,1024,1200,1600\" data-cmp-dmimage=\"\" data-asset=\"\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/BIOFIRE MYCOPLASMA PROTOCOL.jpeg\" data-title=\"&lt;60 min chart\" data-cmp-data-layer=\"{&quot;image-e22390c7e8&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/image&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:44:45Z&quot;,&quot;image&quot;:{&quot;repo:id&quot;:&quot;1593b4a4-86ea-4b1a-be53-f885b1262d3a&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:44:20Z&quot;,&quot;@type&quot;:&quot;image\/jpeg&quot;,&quot;repo:path&quot;:&quot;\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/BIOFIRE MYCOPLASMA PROTOCOL.jpeg&quot;,&quot;xdm:smartTags&quot;:{&quot;template&quot;:0.5208092927932739,&quot;symbol&quot;:0.6541544198989868,&quot;concept&quot;:0.59795081615448,&quot;mass&quot;:0.5248705148696899,&quot;icon&quot;:0.6945244073867798,&quot;sign&quot;:0.6328638195991516,&quot;periodic table&quot;:0.5629279017448425,&quot;button&quot;:0.6064805388450623,&quot;3d&quot;:0.5225829482078552,&quot;computer&quot;:0.5989869832992554,&quot;design&quot;:0.595885694026947,&quot;electronic&quot;:0.5390453934669495,&quot;vector&quot;:0.704307496547699,&quot;communication&quot;:0.5400776267051697,&quot;element&quot;:0.5951201915740967,&quot;business&quot;:0.6707267761230469,&quot;shape&quot;:0.5244226455688477,&quot;weight&quot;:0.5196479558944702,&quot;money&quot;:0.5168244242668152,&quot;background&quot;:0.5373075604438782,&quot;atomic&quot;:0.5495437979698181,&quot;illustration&quot;:0.6087104082107544,&quot;atom&quot;:0.5408551692962646,&quot;internet&quot;:0.5496892929077148,&quot;finance&quot;:0.5402747988700867}}}}\"><img decoding=\"async\" class=\"cmp-image__image\" src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/BIOFIRE%20MYCOPLASMA%20PROTOCOL?qlt=85&amp;wid=1600&amp;ts=1668105885165&amp;dpr=off\" alt=\"&lt;60 min chart\" data-cmp-hook-image=\"image\" \/><\/div>\n<\/div>\n<div class=\"text aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"text-e1f8c77b28\" class=\"cmp-text\" style=\"box-sizing: border-box; font-size: var(--font-text-rg); position: relative; z-index: 1;\" data-cmp-data-layer=\"{&quot;text-e1f8c77b28&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/text&quot;,&quot;repo:modifyDate&quot;:&quot;2023-01-26T09:42:06Z&quot;,&quot;xdm:text&quot;:&quot;&lt;h2&gt;BIOFIRE FILMARRAY 2.0 INDUSTRY SYSTEM&lt;\/h2&gt;\\r\\n&lt;p&gt;The system utilizes a FILMARRAY&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;2.0 Industry instrument and next generation PCR testing in a&amp;nbsp;closed pouch to detect the presence of mycoplasma (Figures 1 and 2). The disposable BIOFIRE&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;Mycoplasma pouch contains all of the necessary reagents for automated cell lysis, nucleic acid&amp;nbsp;purification, reverse transcription, first and second stage nested PCR and analyst detection in order&amp;nbsp;to isolate, amplify and detect over 130 different mycoplasma species (Figure 2).&lt;\/p&gt;\\r\\n&lt;p&gt;&lt;br&gt;\\r\\nSeveral controls are integrated into the pouch to ensure the quality of the results including a total&amp;nbsp;process control, reverse transcription control, and PCR I and PCR II controls. The instrument and&amp;nbsp;software process the pouch with results in less than an hour.&lt;\/p&gt;\\r\\n&lt;p&gt;&lt;br&gt;\\r\\nThe FILMARRAY&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;2.0 Industry software (21 CFR Part 11 compliance ready) performs all of the&amp;nbsp;complex meta-analysis and provides presence\/absence results as either \u201cMycoplasma Detected\u201d&amp;nbsp;or \u201cMycoplasma Not Detected\u201d.&lt;\/p&gt;\\r\\n&quot;}}\">\n<h2><span class=\"ez-toc-section\" id=\"BIOFIRE_FILMARRAY_20_INDUSTRY_SYSTEM\"><\/span>BIOFIRE FILMARRAY 2.0 INDUSTRY SYSTEM<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The system utilizes a FILMARRAY<sup>\u00ae<\/sup>\u00a02.0 Industry instrument and next generation PCR testing in a\u00a0closed pouch to detect the presence of mycoplasma (Figures 1 and 2). The disposable BIOFIRE<sup>\u00ae<\/sup>\u00a0Mycoplasma pouch contains all of the necessary reagents for automated cell lysis, nucleic acid\u00a0purification, reverse transcription, first and second stage nested PCR and analyst detection in order\u00a0to isolate, amplify and detect over 130 different mycoplasma species (Figure 2).<\/p>\n<p>Several controls are integrated into the pouch to ensure the quality of the results including a total\u00a0process control, reverse transcription control, and PCR I and PCR II controls. The instrument and\u00a0software process the pouch with results in less than an hour.<\/p>\n<p>The FILMARRAY<sup>\u00ae<\/sup>\u00a02.0 Industry software (21 CFR Part 11 compliance ready) performs all of the\u00a0complex meta-analysis and provides presence\/absence results as either \u201cMycoplasma Detected\u201d\u00a0or \u201cMycoplasma Not Detected\u201d.<\/p>\n<\/div>\n<\/div>\n<div class=\"text aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--default--0\">\n<div id=\"text-1d5eb05e53\" class=\"cmp-text\" style=\"box-sizing: border-box; font-size: var(--font-text-rg); position: relative; z-index: 1;\" data-cmp-data-layer=\"{&quot;text-1d5eb05e53&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/text&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:47:39Z&quot;,&quot;xdm:text&quot;:&quot;&lt;p&gt;Figure 1:&lt;\/p&gt;\\r\\n&lt;p&gt;FILMARRAY&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;2.0 Industry instrument performs the extraction, amplification and detection (25.4 x 39.3 x 16.5 cm WxDxH). The system comes standard with 2 instruments; up to 8 instruments can be connected to a single PC.&lt;\/p&gt;\\r\\n&quot;}}\">\n<p>Figure 1:<\/p>\n<p>FILMARRAY<sup>\u00ae<\/sup>\u00a02.0 Industry instrument performs the extraction, amplification and detection (25.4 x 39.3 x 16.5 cm WxDxH). The system comes standard with 2 instruments; up to 8 instruments can be connected to a single PC.<\/p>\n<\/div>\n<\/div>\n<div class=\"image aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--default--0\">\n<div id=\"image-336bf44879\" class=\"cmp-image\" data-cmp-lazy=\"\" data-cmp-lazythreshold=\"0\" data-cmp-src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/FILMARRAY%20Industry%20Instrument?qlt=85&amp;wid=%7B.width%7D&amp;ts=1668106038107&amp;dpr=off\" data-cmp-widths=\"320,480,600,800,1024,1200,1600\" data-cmp-dmimage=\"\" data-asset=\"\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/FILMARRAY Industry Instrument.jpeg\" data-title=\"FILMARRAY\" data-cmp-data-layer=\"{&quot;image-336bf44879&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/image&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:47:18Z&quot;,&quot;image&quot;:{&quot;repo:id&quot;:&quot;6cf80b9c-4c01-40c0-8e7a-26b4036e1e9f&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:46:53Z&quot;,&quot;@type&quot;:&quot;image\/jpeg&quot;,&quot;repo:path&quot;:&quot;\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/FILMARRAY Industry Instrument.jpeg&quot;,&quot;xdm:smartTags&quot;:{&quot;electronics&quot;:0.5747535824775696,&quot;color&quot;:0.5650557279586792,&quot;isolated&quot;:0.7385416626930237,&quot;office&quot;:0.6708436012268066,&quot;3d&quot;:0.5699213147163391,&quot;computer&quot;:0.726584792137146,&quot;copier&quot;:0.5613290667533875,&quot;gray&quot;:0.5667309761047363,&quot;white&quot;:0.6969916224479675,&quot;paper&quot;:0.6695451736450195,&quot;laser&quot;:0.5627506375312805,&quot;inkjet&quot;:0.5845353007316589,&quot;digital&quot;:0.5992746949195862,&quot;business&quot;:0.7089658975601196,&quot;printer&quot;:0.762461245059967,&quot;black&quot;:0.5997570157051086,&quot;equipment&quot;:0.7287006378173828,&quot;technology&quot;:0.6321894526481628,&quot;printing&quot;:0.5649793148040771,&quot;ink-jet&quot;:0.5732375979423523,&quot;print&quot;:0.6838370561599731,&quot;close-up&quot;:0.5619229078292847,&quot;background&quot;:0.617030143737793,&quot;machine&quot;:0.5716286897659302,&quot;device&quot;:0.6626167893409729}}}}\"><img decoding=\"async\" class=\"cmp-image__image\" src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/FILMARRAY%20Industry%20Instrument?qlt=85&amp;wid=1024&amp;ts=1668106038107&amp;dpr=off\" alt=\"FILMARRAY\" data-cmp-hook-image=\"image\" \/><\/div>\n<\/div>\n<div class=\"image aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--default--0\">\n<div id=\"image-4c72d17c61\" class=\"cmp-image\" data-cmp-lazy=\"\" data-cmp-lazythreshold=\"0\" data-cmp-src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/BIOFIRE%20MYCOPLASMA%20POUCH%20picture?qlt=85&amp;wid=%7B.width%7D&amp;ts=1668106126478&amp;dpr=off\" data-cmp-widths=\"320,480,600,800,1024,1200,1600\" data-cmp-dmimage=\"\" data-asset=\"\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/BIOFIRE MYCOPLASMA POUCH picture.jpeg\" data-title=\"BIOFIRE MYCOPLASMA\" data-cmp-data-layer=\"{&quot;image-4c72d17c61&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/image&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:48:46Z&quot;,&quot;image&quot;:{&quot;repo:id&quot;:&quot;6e20f33a-7628-4128-86f7-5f1b28448bb1&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:47:23Z&quot;,&quot;@type&quot;:&quot;image\/jpeg&quot;,&quot;repo:path&quot;:&quot;\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/BIOFIRE MYCOPLASMA POUCH picture.jpeg&quot;,&quot;xdm:smartTags&quot;:{&quot;electronics&quot;:0.5671459436416626,&quot;data&quot;:0.5778220295906067,&quot;sound&quot;:0.6721484661102295,&quot;icon&quot;:0.654808759689331,&quot;isolated&quot;:0.6282763481140137,&quot;cassette&quot;:0.5698833465576172,&quot;radio&quot;:0.5965428352355957,&quot;button&quot;:0.6394022107124329,&quot;computer&quot;:0.6017985939979553,&quot;music&quot;:0.7127789258956909,&quot;tape&quot;:0.5705554485321045,&quot;web&quot;:0.5724635124206543,&quot;design&quot;:0.6445760726928711,&quot;electronic&quot;:0.5883170366287231,&quot;vector&quot;:0.640840470790863,&quot;audio&quot;:0.6696782112121582,&quot;digital&quot;:0.6003409624099731,&quot;old&quot;:0.6425942182540894,&quot;retro&quot;:0.6564493179321289,&quot;equipment&quot;:0.6324402689933777,&quot;technology&quot;:0.6210667490959167,&quot;background&quot;:0.6091028451919556,&quot;stereo&quot;:0.5805054903030396,&quot;illustration&quot;:0.5880951881408691,&quot;device&quot;:0.5842410922050476}}}}\"><img decoding=\"async\" class=\"cmp-image__image\" src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/BIOFIRE%20MYCOPLASMA%20POUCH%20picture?qlt=85&amp;wid=1024&amp;ts=1668106126478&amp;dpr=off\" alt=\"BIOFIRE MYCOPLASMA\" data-cmp-hook-image=\"image\" \/><\/div>\n<\/div>\n<div class=\"text aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--default--0\">\n<div id=\"text-48ac3b06e5\" class=\"cmp-text\" style=\"box-sizing: border-box; font-size: var(--font-text-rg); position: relative; z-index: 1;\" data-cmp-data-layer=\"{&quot;text-48ac3b06e5&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/text&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:49:08Z&quot;,&quot;xdm:text&quot;:&quot;&lt;p&gt;Figure 2:&lt;\/p&gt;\\r\\n&lt;p&gt;A. BIOFIRE&lt;sup&gt;\u00ae&lt;\/sup&gt;&amp;nbsp;Mycoplasma pouch.&lt;\/p&gt;\\r\\n&lt;p&gt;B. Pouch diagram: (A) Fitment with freezedried reagents; (B) Plungers-deliver reagents to blisters; (C) Sample lysis and bead collection; (D) Wash; (E) Magnetic bead collection blister; (F) Elution; (G) Multiplex Outer PCR blister; (H) Dilution blister; (I) Inner Nested PCR array&lt;\/p&gt;\\r\\n&quot;}}\">\n<p>Figure 2:<\/p>\n<p>A. BIOFIRE<sup>\u00ae<\/sup>\u00a0Mycoplasma pouch.<\/p>\n<p>B. Pouch diagram: (A) Fitment with freezedried reagents; (B) Plungers-deliver reagents to blisters; (C) Sample lysis and bead collection; (D) Wash; (E) Magnetic bead collection blister; (F) Elution; (G) Multiplex Outer PCR blister; (H) Dilution blister; (I) Inner Nested PCR array<\/p>\n<\/div>\n<\/div>\n<div class=\"text aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"text-e8c8a8008b\" class=\"cmp-text\" style=\"box-sizing: border-box; font-size: var(--font-text-rg); position: relative; z-index: 1;\" data-cmp-data-layer=\"{&quot;text-e8c8a8008b&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/text&quot;,&quot;repo:modifyDate&quot;:&quot;2023-01-26T09:43:10Z&quot;,&quot;xdm:text&quot;:&quot;&lt;h2&gt;SAMPLE PROTOCOLS&lt;\/h2&gt;\\r\\n&lt;p&gt;Two distinct protocols have been designed to detect mycoplasma contamination(3). These include a 0.2 mL direct test that can be used for in-process control testing with a validated LOD of ~\u226430 CFU\/&amp;nbsp;mL, and a 10 mL release test that concentrates the sample using centrifugation and has a validated&amp;nbsp;LOD of \u226410 CFU\/mL.&lt;\/p&gt;\\r\\n&lt;p&gt;&lt;br&gt;\\r\\nThese protocols were followed by evaluators except that Evaluator B deviated from the&amp;nbsp;manufacturer recommendations with customized centrifugation times and forces. Following sample&amp;nbsp;pre-processing, samples were then loaded onto a fully prepared and hydrated pouch and run on the&amp;nbsp;FILMARRAY\u00ae 2.0 Industry instrument.&lt;\/p&gt;\\r\\n&quot;}}\">\n<h2><span class=\"ez-toc-section\" id=\"SAMPLE_PROTOCOLS\"><\/span>SAMPLE PROTOCOLS<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Two distinct protocols have been designed to detect mycoplasma contamination(3). These include a 0.2 mL direct test that can be used for in-process control testing with a validated LOD of ~\u226430 CFU\/\u00a0mL, and a 10 mL release test that concentrates the sample using centrifugation and has a validated\u00a0LOD of \u226410 CFU\/mL.<\/p>\n<p>These protocols were followed by evaluators except that Evaluator B deviated from the\u00a0manufacturer recommendations with customized centrifugation times and forces. Following sample\u00a0pre-processing, samples were then loaded onto a fully prepared and hydrated pouch and run on the\u00a0FILMARRAY\u00ae 2.0 Industry instrument.<\/p>\n<\/div>\n<\/div>\n<div class=\"image aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"image-0bc9be877f\" class=\"cmp-image\" data-cmp-lazy=\"\" data-cmp-lazythreshold=\"0\" data-cmp-src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/Sample%20protocol%20LOD%2033CFU?qlt=85&amp;wid=%7B.width%7D&amp;ts=1668106289781&amp;dpr=off\" data-cmp-widths=\"320,480,600,800,1024,1200,1600\" data-cmp-dmimage=\"\" data-asset=\"\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/Sample protocol LOD 33CFU.jpeg\" data-title=\"sample protocol\" data-cmp-data-layer=\"{&quot;image-0bc9be877f&quot;:{&quot;@type&quot;:&quot;onebmx\/components\/image&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:51:29Z&quot;,&quot;image&quot;:{&quot;repo:id&quot;:&quot;33ff3a72-f087-44e6-a1e0-361b3e1498af&quot;,&quot;repo:modifyDate&quot;:&quot;2022-11-10T18:51:23Z&quot;,&quot;@type&quot;:&quot;image\/jpeg&quot;,&quot;repo:path&quot;:&quot;\/content\/dam\/biomerieux-com\/03----our-offer\/global-resources\/pharma-resources\/Sample protocol LOD 33CFU.jpeg&quot;,&quot;xdm:smartTags&quot;:{&quot;symbol&quot;:0.6084483861923218,&quot;arrow&quot;:0.6222299337387085,&quot;concept&quot;:0.599021315574646,&quot;icon&quot;:0.6974989771842957,&quot;sign&quot;:0.5864791870117188,&quot;button&quot;:0.6256077885627747,&quot;computer&quot;:0.5934608578681946,&quot;download&quot;:0.5872851610183716,&quot;search&quot;:0.5780332684516907,&quot;modern&quot;:0.5710219144821167,&quot;web&quot;:0.6810231804847717,&quot;design&quot;:0.6187219619750977,&quot;vector&quot;:0.6741124987602234,&quot;up&quot;:0.5700666308403015,&quot;tag&quot;:0.5656711459159851,&quot;graphic&quot;:0.5676141977310181,&quot;art&quot;:0.5896478295326233,&quot;green&quot;:0.5795096755027771,&quot;business&quot;:0.6897475719451904,&quot;banner&quot;:0.6055840849876404,&quot;abstract&quot;:0.5913369059562683,&quot;blue&quot;:0.6486618518829346,&quot;background&quot;:0.6620569229125977,&quot;illustration&quot;:0.6099250316619873,&quot;internet&quot;:0.6096782088279724}}}}\"><img decoding=\"async\" class=\"cmp-image__image\" src=\"https:\/\/s7g10.scene7.com\/is\/image\/biomerieux\/Sample%20protocol%20LOD%2033CFU?qlt=85&amp;wid=1600&amp;ts=1668106289781&amp;dpr=off\" alt=\"sample protocol\" data-cmp-hook-image=\"image\" \/><\/div>\n<\/div>\n<div class=\"download aem-GridColumn aem-GridColumn--default--12\">\n<div id=\"download-c0c911746e\" class=\"cmp-download\" data-cmp-data-layer=\"{&quot;download-c0c911746e&quot;:{&quot;@type&quot;:&quot;application\/pdf&quot;,&quot;repo:modifyDate&quot;:&quot;2023-01-26T10:01:52Z&quot;,&quot;dc:title&quot;:&quot;DOWNLOAD THE COMPLETE WHITE PAPER AUTOMATED 1-HOUR MYCOPLASMA TESTING IN BIOPRODUCTION&quot;,&quot;xdm:linkURL&quot;:&quot;\/content\/dam\/biomerieux-com\/03----our-offer\/pharma-resources\/biofire-mycoplasma\/BIOFIRE%20MYCOPLASMA%20TESTING%20BIOPRODUCTION%20JAN%2023.pdf.coredownload.pdf&quot;}}\"><\/div>\n<\/div>\n<p><em><strong>Source: <a href=\"https:\/\/www.biomerieux.com\/corp\/en\/resource-hub\/knowledge\/white-papers\/pharmaceutical-qc-white-papers\/automated-1-hour-mycoplasma-testing-in-bioproduction.html\" target=\"_blank\" rel=\"noopener\">https:\/\/www.biomerieux.com\/corp\/en\/resource-hub\/knowledge\/white-papers\/pharmaceutical-qc-white-papers\/automated-1-hour-mycoplasma-testing-in-bioproduction.html<\/a><\/strong><\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>INTRODUCTION Various pharmacopoeias, including the USP, specify a test for mycoplasma\u00a0 contamination must be\u00a0performed as part of the release testing of a product manufactured in the presence of eukaryotic cells\u00a0(1). Current compendial methods require \u226528 days to generate results creating delays\u00a0 releasing product\u00a0to downstream processes. Alternative conventional nucleic acid testing (NAT) methods are available;\u00a0however, until&#8230;<\/p>\n","protected":false},"author":2,"featured_media":8055,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"rank_math_lock_modified_date":false,"footnotes":""},"categories":[81],"tags":[],"class_list":["post-8043","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/8043"}],"collection":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/comments?post=8043"}],"version-history":[{"count":2,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/8043\/revisions"}],"predecessor-version":[{"id":8045,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/posts\/8043\/revisions\/8045"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media\/8055"}],"wp:attachment":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media?parent=8043"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/categories?post=8043"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/tags?post=8043"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}