Agarose electrophoresis of DNA requires a staining step in order to visualise the DNA fragments.\u00a0 This can be done safely using methylene blue solution.\u00a0 Methylene blue binds to negatively charged DNA making DNA visible as blue bands within agarose following electrophoresis.<\/p>\n
Methylene blue solution is provided as a 10x concentrate.\u00a0 To use, dilute 10x concentrate by mixing one part of methylene blue with nine parts of water (Eg. 100\u00a0ml of 10x concentrate added to 900\u00a0ml water).<\/p>\n
The 1x methylene blue can then be used for staining of DNA bands within agarose gels. Place your Agarose gel into a small, clean gel-staining tray. Pour sufficient 1x methylene blue stain into a suitable tray in order to cover the agarose gel.\u00a0 Allow to stand until DNA bands become visible within the agarose.\u00a0 This can take up to 16 hours (overnight).\u00a0 Once staining is complete, rinse the agarose gel with clean water.\u00a0 Improved resolution of DNA bands can be achieved by allowing the gel to destain in a new clean trays and cover with clean water. For best results leave for approx 20 minutes on a shaker with a slow shaking motion. Changing the water during the time will also improve the destaining action.<\/p>\n
Alternatively, DNA can be stained using 10x methylene blue concentrate.\u00a0 Pour sufficient 10x methylene blue to cover the agarose gel and allow to stand for 5-10 minutes.\u00a0 Once staining is complete, rinse the agarose gel in clean water.\u00a0 Improved resolution of DNA bands can be achieved by allowing the gel to destain in clean water.<\/p>\n
Once your gel has been stained and destained you can visualize your bands by placing the gel on a light table or if this is not available place the gel on a white surface.<\/p>\n","protected":false},"excerpt":{"rendered":"
Concentrated DNA stain which when used in this form samples can be stained within 5-10 minutes<\/p>\n","protected":false},"featured_media":7527,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"rank_math_lock_modified_date":false},"product_cat":[87],"product_tag":[3922,3966,3968],"class_list":{"0":"post-7528","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-other-products","7":"product_tag-cleaver-scientific-en","8":"product_tag-colorimetric-dna-stain-120ml-10x","9":"product_tag-problue-en","10":"yith_product_brand-cleaver-scientific-en","12":"first","13":"instock","14":"shipping-taxable","15":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/product\/7528"}],"collection":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/comments?post=7528"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media\/7527"}],"wp:attachment":[{"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/media?parent=7528"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/product_cat?post=7528"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/gd-1.com\/wp-json\/wp\/v2\/product_tag?post=7528"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}