Analysis of free amino acids have applications of various fields, at the beginning of this diagnosis of inherited metabolic disorders, feeding newborn with a metabolic disorder inherited comes from studies. Furthermore, amino acid analysis in diagnosis problems such as liver and kidney disorders, intestinal microflora disorder, nov problems are performed. 43 amino acids are performed at 7.5 minutes with Jasem quantitative amino acids analysis kit for only one injection. Serum / plasma and urine samples are becoming ready for analysis at 5 min.

Maple-syrup urine disease (MSUD) is an inborn error of metabolism characterized by the inherited deficiency of the branched-chain-2-oxoacid dehydrogenase complex which is required in the metabolism of the branched-chain amino acids. The detection of allo-isoleucine allows for positive identification of patients with MSUD reducing the false-positive neonatal screening results. Chromatographic separation of allo-isoleucine is readily achieved in 11 minutes analysis time with the accurate quantification using same sample.