Correlation to light microscopy and relocation in TEM

An “on-board” integrated wide-field fluorescence microscope (iFLM) allows the same sample area to be observed with light, ion, or electron beams. Specially designed TomoGrids ensure correct cryo-lamellae alignment to the tomographic tilt axis, from initial milling through high-resolution TEM imaging.

The iFLM Correlative System enables fluorescence imaging at the electron/ion beam coincidence point. Switching between iFLM targeting and ion milling can be performed without moving the stage. The 180° tilt capability of the CompuStage allows for imaging of the top and bottom surfaces of the sample, which can be helpful for thick samples.

TomoGrids are specifically made for the cryo-tomography workflow and have two flat sides facing each other. These sides prevent misalignment during loading into the cryo-TEM and always ensure proper orientation of the lamella axis with respect to the TEM tilt axis. With TomoGrids, the entire available lamella area can be utilized for data acquisition.

High-quality lamellae with consistent thickness

The Arctis Cryo-PFIB maintains an ultra-clean working environment for several days, ensuring consistent, high-quality lamella preparation. The plasma ion-beam source can switch between xenon, oxygen, and argon ions, facilitating the production of very thin lamellae with optimized surface quality

PFIB technology enables applications that are not covered by liquid metal ion source (LMIS) FIB systems. For example, it is possible to use the different milling properties of the three available ion beams to produce a high-quality sample while avoiding gallium implantation effects. System enclosure is designed with biosafety in mind and an a heat decontamination solution is optionally available for higher biosafety level labs

The compact sample chamber of the Arctis Cryo-PFIB is specifically designed for cryo-operation. Its reduced volume allows for an exceptionally clean operating environment that minimizes water condensation. This designed-in cleanliness is further enhanced by sample cooling via braids and sample shielding with a dedicated cryo-box, ensuring a working environment that routinely allows for multi-day batch sample preparation.

Automated high throughput and connectivity for cryo-tomography

The Autoloader enables robotic sample loading and unloading for up to 12 grids (TomoGrids or AutoGrids), facilitating transfer to the cryo-TEM while minimizing sample damage and contamination risks. In the new web-based user interface loaded grids are first mapped and inspected. Subsequently, lamella positions are chosen, and milling parameters are defined. Milling work is carried out autonomously. Depending on the specimen, the plasma source enables high milling rates for fast, large-volume material removal.

The Autoloader cassette provides a protected environment for the delicate cryo-lamellae samples. Risky manual sample manipulation steps that could damage or contaminate the samples are largely avoided. The cassette is loaded into a capsule docked to the Autoloader, which can be interchanged between the Arctis Cryo-PFIB and Krios or Glacios Cryo-TEMs.