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SpectraMax® iD3/iD5 – Multi-Mode Microplate Readers

Salzburg multi-user microplate readers with a large touchscreen and NFC functionality

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NFC-based personalization and enhanced touchscreen workflows provide walk-up usability

The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.

With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.

Personalize workflows Personalize workflows

Built-in near-field communication (NFC) functionality can access custom protocols and results with a single tap, saving time better spent on research.

Simplify tasks Simplify tasks

The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.

Reduce background noise Reduce background noise

The optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) detector that reduces background noise for greater sensitivity and a wide dynamic range.

Save time and money with a “Microplate Reader & Washer Bundle”

  • The SpectraMax® Multi-Mode Microplate Reader provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for TRF and FP and more.
  • SoftMax® Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options.
  • AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates.

Kickstart your compliance journey with our “GxP Starter Kit”

For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:

  • SpectraMax iD3/iD5 Microplate Reader are modular and upgradeable with a wide range of high performance capabilities.
  • SoftMax® Pro 7.2 GxP Software – The latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity.
  • SoftMax® Pro Validation Package – Provides the most comprehensive documentation and tools available to validate GxP administrator features, software operation, and analysis functions for microplate reader instrumentation.
  • Software Installation & Training – Our software installation services verify and document that required components are installed to operational specifications.
SpectraMax iD3 Multi-Mode Microplate Reader SpectraMax iD5 Multi-Mode Microplate Reader
General specifications
Read modes Absorbance Fluorescence (top/bottom read) Luminescence (top read) Absorbance Fluorescence (top/bottom read) Luminescence (top/bottom read) Time-Resolved Fluorescence (top/bottom read) Fluorescence Polarization (top/bottom read) HTRF (top/bottom read) HTRF
Wavelength ranges Abs: 230 – 1000 nm FL Ex: 250 – 830 nm FL Em: 270 – 850 nm Lumi: 300 – 850 nm Abs: 230 – 1000 nm
FL Ex: 250 – 830 nm
FL Em: 270 – 850 nm
Lumi: 300 – 850 nm
TRF Ex: 350 nm
TRF Em: 450 – 750 nm
FP: 300 – 750 nm
Wavelength selection Monochromator tunable in 1-nm increments Hybrid filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity < ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV < ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV
Absorbance photometric precision/repeatability < ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV < ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV
Sensitivity top read optimized FL: 1 pM fluoresein 96 well, 1 pM fluorescein 384 well
Lumi: 2 pM ATP 96 well, 4 pM		 FL: Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well; Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well
Lumi: 2 pM ATP 96 well, 4 pM ATP 384 well
TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well
FP: Mono: 2 mP @ 10nM 96 well, 2 mP @ 10nM 384 well; Filters: 1 mP @ 1 nM 96 well, 2 mP @ 1 nM 384 well
Light source(s) High power xenon flash lamp High power xenon flash lamp
Detector(s) Silicon photodiode Ultra-cooled photomultiplier tube Silicon photodiode Ultra-cooled photomultiplier tube
Read types (endpoint, kinetic, etc) Endpoint Kinetic Spectrum scan Well scan Endpoint Kinetic Spectrum scan Well scan
Read times Abs: 28 seconds
FL: 21 seconds
Lumi: 21 seconds		 Abs: 28 seconds
FL: 21 seconds
Lumi: 21 seconds
TRF: 25 seconds
FP: 35 seconds
Plate shaking Linear Orbital Double orbital Linear Orbital Double orbital
Injectors Có Có
Western blot Không Có
Stand-alone touchscreen operation Có Có
Robotics / automation Có
Plate type(s) 6-384 well plates 6-384 well plates
Temperature control Ambient + 5°C to 66°C Ambient + 5°C to 66°C
Dimensions H 40.1 cm x W 53.2 cm x D 59.8 cm H 40.1 cm x W 53.2 cm x D 59.8 cm
Validation tools Absorbance validation plate
Fluorescence validation plate
Luminescence validation plate
SoftMax® Pro GxP Software		 Absorbance validation plate
Fluorescence validation plate
Luminescence validation plate
Multi-mode validation plate
SoftMax® Pro GxP Software

Connectivity Icon QuickSync

The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.

Biểu tượng kiểm soát môi trường Flexible temperature control

Simple-to-use temperature control adjusts the experiment’s conditions from ambient up to 66°C.

Biểu tượng tự động hóa Automatic filter identification

Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.

Biểu tượng bảo mật Enhanced security

The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.

Streamline Icon Hybrid optical system

The SpectraMax iD5 reader provides the flexibility to set up experiments using filters, or a monochromator in 1 nm increments, or a hybrid mix of monochromator and filter-based wavelength selection.

 Flash Assays Icon Flash assays captured with ease

The SpectraMax Injector System with SmartInject® Technology features low dead volume (10 µL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.

Absorbance

Common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

Cell Health

Cell Health

Cell viability refers to the number of healthy cells in a population and can be evaluated using assays that measure enzyme activity, cell membrane integrity, ATP production, and other indicators. These methods can employ luminescent, fluorescent, or colorimetric readouts as indicators of general cell viability or even specific cellular pathways. Cytotoxicity and cell viability assays are often used to assess a drug or other treatment’s effect, and are valuable tools in the search for new therapeutics, as well as advancing our understanding of how normal cells function.

Cell viability

Cell viability

Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.

Cellular Signaling

Cellular signaling allows cells to respond to their environment as well as to communicate with other cells. Proteins located on the cell surface can receive signals from the surroundings and transmit information into the cell via a series of protein interactions and biochemical reactions that comprise a signaling pathway. Multicellular organisms rely upon an extensive array of signaling pathways to coordinate the proper growth, regulation, and functioning of cells and tissues. If signaling between or within cells is dysregulated, inappropriate cellular responses may lead to cancer and other diseases.

DNA/RNA Quantitation

DNA/RNA Quantitation sample

The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation of DNA works on samples ranging from about 0.25 µg/mL to about 125 µg/mL in a microplate format.

Drug Discovery & Development

Drug Discovery & Development Process

For every drug that makes it to the finish line, another nine don’t succeed. This alarming failure rate can be traced to reliance on 2D cell cultures that don’t closely mimic complex human biology, often leading to inaccurate predictions of a drug’s potential and extended drug development timelines.

ELISA

ELISA (Enzyme-linked immunosorbent assays)

Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

Fluorescence

Many fluorescence-based assays include cell viability, GPCR activity, and fluorescent nucleic acid quantification.

Fluorescence Polarization (FP)

Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

HTRF

HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)

IgG Quantification

Immunoglobulin G (IgG) Quantification

In therapeutic protein engineering and cell line development, IgG quantification is the process of measuring the amount of immunoglobulin G (IgG), a common class of therapeutic proteins, produced by a genetically modified cell line. This is important for evaluating and monitoring the productivity of the cell line and selecting the best candidate for further development of therapeutic antibodies.

Luminescence

The key luminescence-based assays include dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

Microbiology and Contaminant

Microbes, including bacteria, have been estimated to make up about 15 percent of the earth’s biomass, and microbes in the human body outnumber human cells by 10 to 1. These microorganisms provide great benefit to us and are also vital to many fields of research from medicine to alternative energy production. On the other hand, monitoring for microbes and the toxic substances they produce is necessary to ensure the safety of pharmaceutical products. Scientists whose research relies on mammalian cells must carefully monitor these cultures for unwanted microbial contaminants to ensure that their experimental results are reliable.

Nucleic Acid (DNA/RNA) Quantitation and Analysis

Nucleic acids are large biomolecules common to all known life forms. Deoxyribonucleic acid (DNA) consists of a double strand of pairs of nucleotides, while ribonucleic acid (RNA) is typically a single strand. In DNA, the nucleotides are adenine, cytosine, guanine, and thymine, while RNA contains uracil instead of thymine. DNA makes up the genetic material of all organisms, encoding the information cells need to synthesize proteins.

Protein Detection, Quantitation and Analysis

Protein detection, quantitation, and analysis are central to the investigation of a wide variety of biological processes. Measuring the concentration of protein is necessary to processes ranging from protein purification and labeling to sample preparation for electrophoresis. Protein can be quantitated directly via absorbance at 280 nm, or indirectly using colorimetric (BCA, Bradford, etc.) or fluorometric methods offering advantages such as greater sensitivity. To identify and measure a specific protein within a complex sample, for example, serum or cell lysate, an ELISA may be used.

Reporter Gene Assay

Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.

TRF, TR-FRET & HTRF

Time-resolved fluorescence – TRF and TR-FRET including HTRF assays provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

Western blotting

Western blotting is a popular technique used for protein detection and quantitation. Molecular Devices introduce a unique Western blot detection system on a multi-mode microplate reader, including colorimetric, fluorescence, and chemiluminescence detection methods.

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